Lab results Flashcards

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1
Q

What would it mean if wound swab came back positive for colliforms?

A

They are colonising flora- not pathological

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2
Q

What do you use to do strep grouping?

A

Lancefield latex test

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3
Q

What would positive surface antigen represent? e.g. of Hep B

A

ongoing or previous infection

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4
Q

What would positive surface antibody represent? e.g. Hep B

A

Immunity

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5
Q

What would core total antibody represent? e.g. Hep B

A

body starting to respond to infection - shows past/current infection

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6
Q

In terms of antibodies and antigens, would would indicate acute infection of Hep B?

A

surface antigen +
core total antibody +/-
surface antibody -

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7
Q

In terms of antibodies and antigens, would would indicate past infection and immunity of Hep B?

A

surface antigen +
core total antibody +
surface antibody +

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8
Q

What rapid test can be done in A&E to check for causes of pneumonia?

A

Strep pneumo + legionella urine test - looks for antigen

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9
Q

What is normal flora on a genital swab?

A

lactobacilli and staph aeureus

- staph a is a worry if from retained tampon, penile swap or if heavy and pure

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10
Q

How do you diagnose UTI in catheter users?

A

not from + dipstick - urine catheters can colonise within 6 hours - must diagnose from symptoms (e.g pyrexia, unwell, confused, increased WCC and CRP)

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11
Q

How would you identify pseudomonas in a culture?

A
  • bacilli
    non-lactose fermenting
    + oxidase
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12
Q

What are some commonalities and differences between staph aureus and epidermidis?

A

both gram + cocci
clusters = staph
+ coagulase = staph aureus (thickens, white, golden clusters on blood agar)
- coagulase = staph epidermidis (white clusters on blood agar)

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13
Q

What do you use to diagnose TB and why?

A

Ziehl-Neelson stain

TB has high myocolic acid in cell wall so is resistant to gram staining due to high lipid content

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14
Q

What is a gram + cocci chain and alpha haemolytic, not sensitive to optichin? Where does it come from and what can it cause?

A

strep viridans
from mouth
can cause endocarditis

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15
Q

What are the results that show H. influenza?

A

gram - bacili

on chocolate agar

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16
Q

What are the bacteria that cause vomiting in gastroenteritis?

A

staph aeureus

bacillus cereus

17
Q

How would you identify enterococci in a culture?

A

gram + cocci

non-haemolytic or beta haemolytic

18
Q

What are the main anti-microbials for gram + cocci?

A

strep - Beta lactams

staph - flucloxicillin

19
Q

What does MRSA stand for? How would you treat it?

A

methicillin resistant staphlococcus aureus

treat with vancomycin

20
Q

What is the most common cause of meningitis in healthy individuals and how do you identify it?

A

neisseria meningitidis
gram - diplococcus
no haemolysis on BA
rash

21
Q

What are the types of gastroenteritis and the main cause?

A

food related - salmonella, campylobacter, staph aureus, bacillus careus, clostridium perifringens, E. coli
infectious - shigella, vibriocholerae, viral (SRV, rotavirus)
antibiotic related - C. difficile

22
Q

What is the most common type of fungus?

A

candida

23
Q

What is the broad spectrum treatment for meningitis?

A

cefotaxime

24
Q

How would you treat viral meningitis once identified?

A

acyclovir

25
Q

What identifies S. pneumoniae?

A

gram + coccus, chains
alpha haemolysis (green)
sensitive to optochin

26
Q

How do you identify viruses and what are the pros and cons?

A

PCR - polymerase chain reaction
fast, v. sensitive
but you need to know the virus you are looking for

27
Q

What is the order of antibody appearance?

A

IgM first and trials off

IgG peaks and appears later but stays up for life

28
Q

Give an example of Lancifield group D enterococci

A

enterococci fecalis

29
Q

What are the 4 stages of gram staining?

A

crystal violet - iodine - decolourisation (+ keeps purple stain) - counter stain (fuchsin, - shows pink)