Lab Quiz/Practical #1

Question 1

The 3 elements of safe containment of microorganisms

Answer 1

1. good laboratory practices and technique
2. safety equipment
3. facility design

Question 2

biological safety cabinet (BSC)

Answer 2

Used as a primary barrier against exposure to infectious biological agents
Has High Efficiency Particulate Air (HEPA) filters
Laminar airflow
NOT chemical fume hoods

Question 3

Containment

Answer 3

refers to safe methods for managing infectious material in the laboratory environment

Question 4

BSL1 containment

Answer 4

Suitable for work involving well-characterized agents not known to cause disease in healthy adult humans and of minimal potential hazard to lab personnel and the environment

No special design features

Question 5

How to determine how many bacterial cells are in the culture

Answer 5

Perform a Quantitative Plate Count

Question 6

Quantitative Plate Count

Answer 6

A series of dilutions are performed so that a modest # of cells can be transferred to an agar plate and colonies counted after incubation

Question 7

1 colony aries from…

Answer 7

1 bacterial cell

Question 8

Dilution schemes are often set up using factors of …

Answer 8

10

Question 9

Individual dilution factors (IDF’s)

Answer 9

represent dilution due to a single transfer

Question 10

total dilution factor (TDF)

Answer 10

Multiply all the IDF’s together
Exponents are added
Possible b/c the base is the same value (10)

Question 11

simple stain

Answer 11

purpose: visualize bacteria
color: methylene blue

Question 12

heat fix

Answer 12

Kills the bacteria

Adheres bacteria to slide because liquids will be applied to slide

Question 13

Condenser

Answer 13

bends and focuses the light onto the stage

Question 14

Iris

Answer 14

controls the amount of light allowed through the microcope