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Microbiology > Lab Quiz 3 > Flashcards

Lab Quiz 3 Flashcards

1
Q

What is the substrate for Starch test?

A

starch

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2
Q

What type of enzyme for starch test?

A

extra-cellular/exoenzyme

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3
Q

What enzyme is involved for starch test?

A

amylase

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4
Q

what is the product for starch test?

A

glucose

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5
Q

what is a positive test result for starch test?

A

clear zone around bacterial growth after addition of iodine

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6
Q

What is the substrate for neutral red fat agar test?

A

lipid (fat)

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7
Q

what type of enzyme for neutral red fat agar test?

A

extracellular/exoenzyme

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8
Q

what enzyme is involved for neutral red fat agar test?

A

lipases

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9
Q

What is the product for neutral red fat agar test?

A

glycerol + fatty acids

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10
Q

What is a positive test result for neutral fat agar test?

A

red color in medium under fat drop

Negative is nothing there

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11
Q

What is the substrate of Gelatin test?

A

gelatin

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12
Q

what type of enzyme for Gelatin test?

A

extracellular/ exoenzyme

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13
Q

what enzyme is involved for Gelatin test?

A

gelatinase

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14
Q

what is the product for Gelatin test?

A

amino acids

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15
Q

what is a positive test result for Gelatin test?

A

Liquefaction of gelatin

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16
Q

What are the substrates for Carbohydrate Fermentation: Glucose, Lactose, and Mannitol test?

A

Glucose, Lactose, Mannitol

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17
Q

What type of enzyme for Carbohydrate fermentation test?

A

Intracellular/endoenzyme

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18
Q

what enzyme is involved for Carbohydrate fermentation test?

A

glycolysis/fermentation

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19
Q

what is the product for Carbohydrate fermentation test?

A

pyruvic acid

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20
Q

what is a positive test result for Carbohydrate fermentation test?

A

red is no acid
yellow is positive for acid
no bubble (gas) is negative
bubbles is positive

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21
Q 
Carbohydrate Fermentation: 
                Glucose, Lactose, and Mannitol Broth 
E. Coli
M.luteus 
P.vulgaris 
S.aureus
A

Carbohydrate Fermentation:
Glucose, Lactose, and Mannitol Broth
E. Coli AG AG AG
M.luteus — —– —-
P.vulgaris A —– —-
S.aureus A A A

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22
Q

What is the substrate for Indole test?

A

tryptophan

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23
Q

What type of enzyme for Indole test?

A

intracellular/ endoenzyme

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24
Q

what type of enzyme is involved for Indole test?

A

tryptophanase

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25
Q

what is the product for Indole test?

A

indole

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26
Q

What is the positive test result for Indole test?

A

red color after adding Kovac’s reagent
Positive: pink layer (E.coli)
Negative: no pink layer (E. aerogenes)

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27
Q

What is the substrate for Methyl Red test?

A

glucose

28
Q

what type of enzyme for Methyl Red test?

A

intracellular/endoenzyme

29
Q

What type of enzyme is involved for Methyl Red test?

A

variable

30
Q

What is the product for Methyl Red test?

A

mixed acids

31
Q

What is a positive test result for Methyl Red test?

A

Methyl red stays red
E.coli was positive
E. aerogenes was negative

32
Q

What is the substrate for Voges/Proskauer (VP) test?

A

Glucose

33
Q

what type of enzyme for VP test?

A

intracellular/endoenzyme

34
Q

what enzyme is involved for VP test?

A

N/A

35
Q

what is the product for VP test?

A

acetylmethyl carbinol

36
Q

what is a positive test result for VP test?

A

red or pink after addition of alpha naphthol + KOH
Negative (E.coli)
Positive (E.aerogenes)

37
Q

what is the substrate for Citrate test?

A

Citrate: Ammonioum Phosphate

38
Q

what type of enzyme for Citrate test?

A

intracellular

39
Q

what enzyme is involved for Citrate test?

A

Citrate permease

N/A

40
Q

What are the products for Citrate test?

A

NH3 or NH4

41
Q

what is a postive test for Citrate test?

A

Citrate permease allows cell to absorb citrate. Citrate is only C source thus allowing bacteria to grow. As they grow, they us (NH4)H2PO4 and pH increases. Green tube turns to blue.
Positive is blue ( E.coli)
negative is not blue ( E. aerogenes)

42
Q

What is a substrate for Hydrogen sulfide test?

A

cysteine or inorganic sulfur compounds

43
Q

what type of enzyme for Hydrogen sulfide test?

A

intracellular/endoenzyme

44
Q

what enzyme is involved for Hydrogen sulfide test?

A

cysteine desulfurase or thosulfate redcutase

45
Q

what is the product for Hydrogen sulfide test?

A

H2S gas (reacts with FeSO4 yield FeS black ppt)

46
Q

what is a positive test result for Hydrogen Sulfide test?

A

Black precipitate
Positve P. vulagris
Negative E.coli

47
Q

What is the substrate for Urease test?

A

urea

48
Q

what type of enyzme for Urease test?

A

intracellular/endoenzyme

49
Q

what enyzme is involved for Urease test?

A

urease

50
Q

what is a product for Urease test?

A

ammonia

51
Q

what is a positive test result for Urease test?

A

postive Fushia color hot pink

52
Q

result for IMViC test for E.coli and Enterobacter aeorgenes

A

I M Vi C
E.coli + + – –
E. aerogenes – — + +

53
Q

For Catalase experiment what are we testing for?

A

Presence of the enzyme catalase

54
Q

What reaction does catalase catalyze?

A

converts hydrogen peroxide into water and gaseous oxygen

55
Q

which groups of organism posses catalase ?

A

B. subtilis

bacillus

56
Q

what does superoxide dismutase do?

A

catalyzes conversion of superoxide radicals to hydrogen peroxide

57
Q

why is oxygen toxic to strict/obligate anaerobes?

A

Strict anaerobic organisms die in the presence of oxygen because the presence of oxygen causes them to create superoxide. Superoxide is toxic and is dismantled in aerobes and facultative anaerobes by the presence of superoxide dismutase. However, strict anaerobes do not have this enzyme, leaving the cell with an accumulation of superoxide which will kill the organism.

58
Q

In the Nitrate Reduction experiment what enzyme we looking for?

A

Nitrate reductase

59
Q

what is Nitrate?

A

medium

60
Q

what will we test for?

A

presence of nitrite.

61
Q

What does Nitrate reductase do?

A

catalyzes the conversion of nitrate to nitrite

62
Q

what color is positive for nitrate?

A

red color

63
Q

If it red before zinc?

A

positive

64
Q

if it red after zinc?

A

negative

65
Q

No color after zinc?

A

positive

66
Q

If nitrate reduced completely what is it?

A

ammonia or nitrogen gas which are super reducers.

Microbiology (11 decks)

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