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Microbiology > Lab Quiz 2 > Flashcards

Lab Quiz 2 Flashcards

1
Q

What do Negative stains do?

A

Negative stain does not stain organism but stains the backgrounds.

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2
Q

how does a capsule appear in prepared slide?

A

clear surrounded by red stained background

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3
Q

Why is negative stained used?

A

to determin morphology and cellular arrangment in bacterica that are too delicate to withstand heat-fixing. (for example Treponema)

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4
Q

What color indicates bacteria is present in the agar? (Motility)

A

Red(TTC- Help us to visualize the bacterial in the agar)

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5
Q

What does the red(TTC) color repersent? (Motility)

A

bacterial metabolism

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6
Q

When oxidized what color is TTC?

A

colorless

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7
Q

When TTC is reduced? What does it mean?

A

it is red and forms an insoluble precipitate.

wherever you see red the bacterial are there.

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8
Q

What does TTC stand for ?

A

Tetrazolium chloride

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9
Q

What are wet mounts used for?

A

allow obersvation of living cells to determin motility. Also used to see natural cell size, arrangement, and shape

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10
Q

What is motility?

A

Move ones self

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11
Q

If it not motility, What is it?

A

Brownian movement (vibrating)

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12
Q

Two type of pigment for slants?

A

Intracellular( on top agar)

Extacellular (inside agar)

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13
Q

Two type of consistency for slants?

A

Mucoid (smooth and shiny)

Dry ( friable , crusty, and cracky)

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14
Q

Four type of growth patterns for broth?

A

Pelllicule (on top)
Tubridity (cloudy
sediment (at the bottom of the meduim tube)
Flaccutant(clump on side of tube or in agar)

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15
Q

What are growth patterns used for?

A

can provide useful information when attempting to identity an organism.

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16
Q

What are common protoza found in lakes and ponds?

A

Amoeba, Paramecia, Euglena, Rotifers, and Volvox

17
Q

What are the three kinds of locomtion for different protoza?What protoza uses them?

A

cilia (paramecia)
Flagella (Euglena)
pseudopods (Amoeba)

18
Q

What can Protozoa cause?

A

Diseasess

19
Q

What does Trypanosoma cause? what class it is in? and What is it pigment?

A

African sleeping sickness, superclass Mastigophora, and extracellular

20
Q

What does Plasmodium cause? what class it is in? and what is it pigment?

A

Malaria in humans and animals, superclass sporoza and intracellular.

21
Q

What is the locomotion of Subphylum ciliophora?

A

ciliates

22
Q

What is the locomotion of Subphylum Sporoza?

A

apicomplexa

23
Q

what is the locomotion of Subphylum Sarcomastgophora?

A

Flagellates and amoebas

24
Q

What is the locomotoion of superclass Mastigophora?

A

Flagellates

25
Q

what is the locomotion of superclass sarcodina?

A

amoebas

26
Q

What is EMB? What are the colors?

A

Eosin Methylene Blue Agar
Shiney green = E.Coli because love lactose
Dark purple = lactose fermented
Light purple/Lavander and sometime blue = No lactose fermented
the sugars provide fermentable substrates to encourage growth of fecal coliforms

27
Q

What is MAC? What are the colors?

A

MacConkey Agar
Pink = lactose fermentation
Not pink= no lactose fermentation
Acid accumulating from lactose fermentation turns the dye red
Crystal violet allow growth of Enterococcus and some species of Staphylococcus, which ferment the lactose and appear pink on the medium

28
Q

What is MSA? What are the colors?

A

Mannitol Salt Agar
Pink growth doesnt not change color of meduim
(staphylococci) (NMF)
YELLOW AGAR ( staphlococcus) MF (Mannitol fermented)

29
Q

What is PEA?

A

Phenylethy Alcohol Agar

allows gorwth of gram postivie organism and stop or inhibit growth of most gram negative organism.

30
Q

What is selective medium?

A

inhibiting growth of some organisms while encouraging the gorwth of others.

31
Q

What is differential media?

A

indcators to expose differences between organisms

32
Q

Can particular meduim may be both selective and differental?

A

YES

33
Q

Describe how a GasPak anaerobic system generates an anaerobic environment?

A

removing the oxygen by CO2 enriched conditions

34
Q

How can you tell wheather an anaerobic enviroment has been achieved in the jar?

A

the blue strip is no longer blue

35
Q

Which organism ares facultative anaerobes?

A

E.coli and B.cereus but E.coli grows better with oxygen

36
Q

which organism are obliagte aerobes?

A

M. Luteus must need air to grow

37
Q

which organism are obliageted anarobes?

A

Clostridium Sporogenes can not tolerate air

Microbiology (11 decks)

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