Lab Practicum Flashcards
Glucose A/A LIA P/P
Indole + Citrate =
Urea =
E. coli
Causes pneumonia and meningitis
Hemophilus influenzae
Gram + pleomorphic rods in parallel lines, Ks, and Vs
Orange growth on chocolate
Normal skin flora
Corynebacterium sp or diptheroids
Gram = coccobacillary; TSI K/K; Motility=; OF Glucose+; Maltose=; Oxidase =
Acinetobacter
If you have suspected S. aureus from yellow MSA plate, what test should you do to confirm?
Coag test should be performed, clot confirms S. aureus
Phenylalanine + genera
Proteus
Providencia
Morganella
Sputum smear shows Gram + lancet shaped diplococci
A disc +
P disc +
Streptococcus pneumoniae
A disc contains bacitracin and contains zone for
Group A Beta Strep
Grey and mucoid
Growth only around XV on Mueller Hinton plate
Tiny Gram = pleomorphic rods
Hemophilus influenzae
Dry pink, >100 colonies on MacConkey indicates
UTI
SBA showing streaks for isolation but only little dot growth in one line
Hemophilus influenzae, called the satellite phenomenon
Camp test +
Group B Beta Streptococcus
What test should be done first if you see Gram + cocci
catalase test
Causes pneumonia, TSS, boils carbuncles
Staphylococcus aureus
Yellow growth on XLD (red)
Lactose fermenter
Yellow growth on MSA
Staphylococcus aureus
Mucoid growth; A/A; Citrate+; Motility=; Ornithine=; Indole=
Klebsiella pneumoniae
Swarming growth on chocolate
Swarming growth on SBA
CNA =
Clear growth on MacConkey
Indole =
Proteus mirabilis
Mucoid growth; A/A; Citrate +; Motility =; Ornithine =; Indole +
Klebsiella oxytoca
If you have a wound culture with growth on SBA, chocolate, and a pink colony on MacConkey, what should you identify
the pink colony
EMB with green metallic sheen
E. coli
P/P on LIA means
lysine +
Swarming growth on SBA
Swarming growth on chocolate
CNA =
Clear growth on MacConkey
Indole +
Proteus vulgaris
TSI A/A; Whitish gray with very narrow zone of β hemolysis; BEA + (black); Motility + (umbrella); Gram + rods
Listeria
>100,000 Gray, moist, non-hemolytic on SBA
MacConkey =
BEA + (black)
NaCl +
Group D Enterococcus
Clear growth on MacConkey; Oxidase+; GNF red; 42P+; Blue/metallic; Most commonly isolated nonfermentor
Pseudomonas aeruginosa
Neutrophils with Gram = coffee bean shaped diplococci intracellularly; Gray colones on Chocolate and Thayer Martin plates
Dextrose + Lactose = Maltose = Sucrose=
Neisseria gonnorhaea
Clotted Coag Hemolysis on SBA
Cream colored colonies on Chocolate agar
Hemolysis on CNA
MacConkey =
Gram + cocci clusters
Staphylococcus aureus
Gram +
Spores
Hemolytic on SBA
Bacillus
TSI K/A with H2S (red/yellow)
Salmonella
GNF tube yellow at butt
ferments glucose
Orange growth on HE (green)
Lactose fermentor
