lab practical exam Flashcards

1
Q

staph aureus

A
SBA- hemolytic
CNA- growth for gram +
gram + cocci slide
coag: clotted
causes: wound infection, throat pneumonia, boils
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2
Q

hemophilus influenza

A
sputum no growth on SBA
gray mucoid on chocolate
plate with 4 streaks, grows best on XV disc
slide with tiny pleomorphic rods gram =
causes meningitis
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3
Q

E. Coli

A
urine sample with dry pink colonies on mac. 
>100 colonies = UTI
TSI: A/A
LIA P/P
indole +
CIT =
UREA =
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4
Q

neisseria gonorrhea

A

slide gram = diplococci “coffee bean” shaped bacteria inside neutrophils
choco. and thayer martin grow gray colonies
dextrose (glucose) +
oxidase +

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5
Q

strep pneumoniae

A

slide from sputum is gram + (purple) lancet shaped with two segments, flattened, and pointed ends
SBA plate with “P” disc alpha hemolytic colonies

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6
Q

CAMp test

A

sheep plate containing a staph streak growing up to an arrow - identifies Group B Beta Strep

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7
Q

Group D Enterococcus

A

SBA will be gray moist nonhemolytic colonies
MAC plate with no growth
bile esculin + (black)
salt broth + (cloudy)

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8
Q

how to report out culture on sputum plate?

A

deciding if this is normal flora, if there are no accompanying test then it is saying it is indeed normal flora

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9
Q

how to report out a stool plate?

A

CNA - any hemolytic colonies are pathogenic
MAC- will be pink for normal flora
HE- orange
XLD- yellow

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10
Q

proteus

A

SBA & CHOC will have swarming colonies
CNA & MAC no growth
morphology for swarming is swarming motility

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11
Q

how to differentiate between Mirabilis and Vulgaris

A

M is indole = and ornithin + (purple)

V is indole + (red) ornithin = (yellow)

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12
Q

klebsiella

A
very mucoid plate
TSI A/A 
citrate +
motility =
ornithin =
LIA +
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13
Q

how to differentiate between K. pneumoniae and K. oxytoca

A

indole
P is =
O is +

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14
Q

corynebacterium

A

blood culture on a chocolate plate > have bright orange colonies
slide will have gram + plemorphic rod with “picket fence” or “chinese letter” shapes
explanation for why its in blood: dipthroid disease or staph epi contamination

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15
Q

pseudomonas

A
urine culture 
green metallic colonies on SBA
clear on MAC
oxidase + 
GNF tube is red (non fermentor)
42 degree tube has growth
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16
Q

pathogenic salmonella

A

TSI tube with K/A H2S (red on slant yellow but)

17
Q

pseudomonas aerogenosa

A

most commonly isolated non fermentor

18
Q

acetobacter

A
second most commonly isolated nonfermentor
TSI K/K
blood and mac plate
glucose +
maltose +
motility = 
oxidase =
19
Q

listeria

A
whitish - gray beta hemolytic SBA
TSI A/A 
bile esculin +
motility at 37 =
motility at 25 umbrella +
slide gram + rods
20
Q

Group A Beta Strep

A

sub plate from throat with zone around “A” disc
can tell on MIO with kovac’s
“A” disc contains bacitracin

21
Q

bacillus

A

slide with gram + with spores

SBA flat and hemolytic

22
Q

phenylalanine

A

+ = blue on slant and/or growth on slant\

three genera providencia, proteus, morganella

23
Q

nitrate broth

A

red after use of reagents A and B = + result
however, if clear use zinc dust
stays clear =

24
Q

if there is a gram = rod in blood culture, when given a sample of the culture what would you need to do?

A

the wound has growth on sheep and choc. and they’re pink colonies on MAC
answer question as “ID growth on MAC” etc wth XLD and HE

25
what's the first test to do on gram +?
catalase
26
what does it mean when a mannitol salt agar turns yellow?
staph aureus confirm with coagulase
27
when looking at LIA agar how would you read P/P with black
H2S + | LIA +
28
HE medium is green
nonfermentor
29
orannge HE medium
lactose fermentor
30
black HE medium
H2S +
31
yellow XLD medium
lactose fermentor
32
NLF on XLD medium
reddish
33
EMB with green metallic sheen
E. Coli
34
a SBA plate with staph streak will have colonies growing around the staph streak what does this demonstrate and what organism is present?
satellite phenomenon | hemophilus influenza
35
what does it mean if GNF tube is yellow?
glucose fermentor