lab practical exam Flashcards
staph aureus
SBA- hemolytic CNA- growth for gram + gram + cocci slide coag: clotted causes: wound infection, throat pneumonia, boils
hemophilus influenza
sputum no growth on SBA gray mucoid on chocolate plate with 4 streaks, grows best on XV disc slide with tiny pleomorphic rods gram = causes meningitis
E. Coli
urine sample with dry pink colonies on mac. >100 colonies = UTI TSI: A/A LIA P/P indole + CIT = UREA =
neisseria gonorrhea
slide gram = diplococci “coffee bean” shaped bacteria inside neutrophils
choco. and thayer martin grow gray colonies
dextrose (glucose) +
oxidase +
strep pneumoniae
slide from sputum is gram + (purple) lancet shaped with two segments, flattened, and pointed ends
SBA plate with “P” disc alpha hemolytic colonies
CAMp test
sheep plate containing a staph streak growing up to an arrow - identifies Group B Beta Strep
Group D Enterococcus
SBA will be gray moist nonhemolytic colonies
MAC plate with no growth
bile esculin + (black)
salt broth + (cloudy)
how to report out culture on sputum plate?
deciding if this is normal flora, if there are no accompanying test then it is saying it is indeed normal flora
how to report out a stool plate?
CNA - any hemolytic colonies are pathogenic
MAC- will be pink for normal flora
HE- orange
XLD- yellow
proteus
SBA & CHOC will have swarming colonies
CNA & MAC no growth
morphology for swarming is swarming motility
how to differentiate between Mirabilis and Vulgaris
M is indole = and ornithin + (purple)
V is indole + (red) ornithin = (yellow)
klebsiella
very mucoid plate TSI A/A citrate + motility = ornithin = LIA +
how to differentiate between K. pneumoniae and K. oxytoca
indole
P is =
O is +
corynebacterium
blood culture on a chocolate plate > have bright orange colonies
slide will have gram + plemorphic rod with “picket fence” or “chinese letter” shapes
explanation for why its in blood: dipthroid disease or staph epi contamination
pseudomonas
urine culture green metallic colonies on SBA clear on MAC oxidase + GNF tube is red (non fermentor) 42 degree tube has growth
pathogenic salmonella
TSI tube with K/A H2S (red on slant yellow but)
pseudomonas aerogenosa
most commonly isolated non fermentor
acetobacter
second most commonly isolated nonfermentor TSI K/K blood and mac plate glucose + maltose + motility = oxidase =
listeria
whitish - gray beta hemolytic SBA TSI A/A bile esculin + motility at 37 = motility at 25 umbrella + slide gram + rods
Group A Beta Strep
sub plate from throat with zone around “A” disc
can tell on MIO with kovac’s
“A” disc contains bacitracin
bacillus
slide with gram + with spores
SBA flat and hemolytic
phenylalanine
+ = blue on slant and/or growth on slant\
three genera providencia, proteus, morganella
nitrate broth
red after use of reagents A and B = + result
however, if clear use zinc dust
stays clear =
if there is a gram = rod in blood culture, when given a sample of the culture what would you need to do?
the wound has growth on sheep and choc. and they’re pink colonies on MAC
answer question as “ID growth on MAC” etc wth XLD and HE
what’s the first test to do on gram +?
catalase
what does it mean when a mannitol salt agar turns yellow?
staph aureus confirm with coagulase
when looking at LIA agar how would you read P/P with black
H2S +
LIA +
HE medium is green
nonfermentor
orannge HE medium
lactose fermentor
black HE medium
H2S +
yellow XLD medium
lactose fermentor
NLF on XLD medium
reddish
EMB with green metallic sheen
E. Coli
a SBA plate with staph streak will have colonies growing around the staph streak what does this demonstrate and what organism is present?
satellite phenomenon
hemophilus influenza
what does it mean if GNF tube is yellow?
glucose fermentor
