Lab Practical 1 Flashcards
Organization of the body
Cells –> tissues –> organ –> systems
Tissue Definition
a grouping of similar cell types with similar functions that eventually make up the structure of organs (groups of tissues)
Epithelial Tissue Definition
lines voids (cavities, organs, or a body); protection, selectively permeable barrier, sensory surfaces, secretion, regenerative, closely packed, polarity (one attached surface [basement membrane, which stains dark] where the cells grow outward from)
Epithelial Tissue Types
Simple Squamous
Simple Cuboidal
Simple Columnar
Pseudostratified Columnar
Stratified Squamous
Simple Squamous
EPITHELIUM
- Found in a single layer, irregular, flattened shape
- Function: Secretion of lubricating substances
Location: Air sacs (alveoli) in lungs; more geometric shape & note capillaries


Simple Sqamous
EPITHELIUM
Simple Cuboidal
EPITHELIUM
- Single layer, cube like
- central nuclei looks like a baloon in a box
- job: secretion + absorption
- Location: kidney tubules, small gland ducts


Simple Cuboidal
EPITHELIUM
Simple Columnar
EPITHELIUM
- Found in a single layer with round/oval nuclei; taller than they are wide
- Job: absorb, secrete mucus… can also have microvilli
- Location: digestive tract lining


Simple Columnar
EPITHELIUM
Pseudostratified Columnar
EPITHELIUM
- Found in a single layer of cells that appears to be multiple layers due to height variance and location of nuclei
- May contain goblet cells and bear cilia
- Job: secrete mucus
- Location: trachea


Pseudostratified Columnar
EPITHELIUM
Stratified Squamous
EPITHELIUM
- Flat cells in more than 1 layer
- Job: protect underlying tissues
- Location: epidermis


Stratified Squamous
EPITHELIUM
Connective Tissue Definition
“cells embedded in a matrix;” protect (defense), structure, & bind together (connective tissue after epithelial to connect to muscle)
Connective Tissue Types
Loose Connective (LC)
LC, Adipose
Hyaline Cartilage
Bone
Monocyte (blood)
Neutrophil (blood)
Lymphycyte (blood)
RBC/ Erythrocytes (blood)
Platelets (blood)
Loose Connective Tissue
CONNECTIVE TISSUE
- gel like matrix with a jumble of fibers
- job: wrap and cushion
- location: under skin


Loose Connective Tissue
CONNECTIVE TISSUE
Adipose Tissue (LC)
CONNECTIVE TISSUE
- Large, round vacuoles (adipose [fat] cells) that have nucleus pushed to the side by large fat droplet
- job: energy storage, support/protect organs
- Location: Aveolar connective tissue, subcutaneous tissues, bone marrow; fat


CONNECTIVE TISSUE
Adipose
Hyaline Cartilage
CONNECTIVE TISSUE
- Smooth, amorphous matrix (that will appear uniformly stained) with cells suspended in it (lacunae and chondrocytes)
- Job: structure and support
- Location: joints, trachea


CONNECTIVE TISSUE
Hyaline Cartilage
Lacunae
Chambers in hyaline cartilage that contain chondrocytes
Chondrocytes
Located in the lacunae in cartilage
Secrete matrix
Lamellae
Concentric rings of bone
Contain Haversian Canals
Haversian Canals
Canal in the center of bone that allow nerves and blood vessels to reach osteocytes
Osteocytes
Sit in lacunae and secrete bone matrix
Canaliculi radiate out of them
Canaliculi
Tiny channels radiating out of osteocyte lacunae
Connect cells with blood supply

Bone
CONNECTIVE TISSUE
Monocyte
CONNECTIVE TISSUE - Blood
- Kidney shaped nucleus
- Does immune surveillance


Monocyte
CONNECTIVE TISSUE - Blood
Neutrophil
CONNECTIVE TISSUE - Blood
- Chunks of nucleus
- Does immune defense


Neutrophil
CONNECTIVE TISSUE - Blood
Lymphocyte
CONNECTIVE TISSUE - Blood
- Whole cell = nucleus
- Stains purple
- Immune Response


Lymphocyte
CONNECTIVE TISSUE - Blood
Red Blood Cells
CONNECTIVE TISSUE - Blood
- No nucleus
- Gas exchange


Red Blood Cell
CONNECTIVE TISSUE - Blood
Platelets
CONNECTIVE TISSUE - Blood
- Smaller than RBC
- Stain as little purple dots
- Job: clotting
Muscle Tissue
contractile tissue, produces movement
Types
- Cardiac
- Smooth
- Skeletal
Skeletal Muscle
- Voluntary
- Striated
- Tubular thick and thin filaments that are regularly attached, causing it to have vertical lines; multinucleated, where nuclei are elongated and pushed to the sides


Skeletal Muscle
MUSCLE
Cardiac Muscle
- Involuntary
- Striated
- Lots of intercalated discs
- Branched


Cardiac Muscle
MUSCLE
Smooth Muscle
- Involuntary
- No striations
- One nucleus


Smooth Muscle
MUSCLE

Blastula, Fate Map
Light Blue: Ectoderm
Dark Blue: Ecotoderm –> Nervous Tissue
Red: Mesoderm
Green: Mesoderm –> Notochord
Yellow: Endoderm

Cross Section of Blastula
- White Space = blastocoel
Top = Animal Pole
Bottom = Vegetal Pole

Early Gastrulation
Green = Dorsal Lip of blastopore
- Movement of cell into 3 germ layers has begun

Gastrulation
Forming of archenteron (white line pointing to it)

More Gastrulation
Expansion of arhcenteron

- Blastocoel (white) pushed aside by expansion of arch…. eventually disapears
- Archenterone lined with endoderm

Completed Gastrulation
- Blastopore plug at beginning of arch. –> anus
- 3 germ layers est.
- Green differentiating into notochord

- Green notochord (from meso)
- Dark Blue ectoderm differentiate into neural tube (induction from notochord)

Neurulation - Longitudinal Section
- Fully closed neural tube
- Anus where blastopore was
- Gut lined with endoderm (in yellow)

Neural Crest Migration
- Open gut and anus
- Red meso will be muscle
- Dark red = developing heart and other tissues
- Noto will be replaced with bone
- Nerve cord expands to form brain
Lable: 1-6

Human Ovum and Sperm
- 2 haploid cells
1. Corona Radiata
2. ZP
3. Egg Cytoplasm
4. Egg Nucleus
5. Sperm
6. First polar body
Label 1-5

Sperm Enters
- ZP
- Egg cortex with granules
- Egg cytoplasm
- Egg pronucleus
- First folar body
Label 1-4

Meiosis II Spindle
- Egg chromosome on splindle that is about to complete meiosis II
- Spindle MT
- Sperm pronucleus
- First polar body
Label 1-3

Both Pronuclei Present
- Egg Pro.
- Sperm Pro.
- Both Polar Bodies
Label 1-4

Pre-Nuclei Fusion
- Egg pro. with haploid set of chromosomes
- Sperm pro. with haploid set of chromosomes
- Poles forming for first mitotic division
- Both polar bodies
What do we call this?
What process led to formation?
Hap or dip?

Zygote
From fusion of pronuclei
Dipoloid
Name the developmental process that has begun.
Give 2 features of this process in deuterostomes.

- Cleavage
- Radial and splits before growth
of cell divisions
type of cell division

- 2
- Mitosis
Label 1-3
Talk about X chromosomes if female

16 Cell Stage
- Centrosomes
- Interphase nuclei of blastomere
- Polar bodies
X inactivation!
Label 1-8

Early Implantation
- Primary wall blastula
- Blastocoel
- iWill be primary endoderm
- Bulk of embryo proper
- Start of amniotic cavity
- Trophoblast cells
- Vascularlized endometrium
- Endometrial epithelium
Label: 1,2,3,4,9,10

15 Days Old
- Epiblast (ectoderm)
- Amnion
- Endoderm
- Yolk (vitelline sac)
- Trophoblast (chorion) cells
- Maternal Blood Island
Label: 1-3, 5, 6, 7, 10

3rd Week
- Brain forming
- Notochord
- Endoderm
- Amniotic Cavity
- Yolk Sac
- Early Heart
- Chorionic Villi
Label 1-4, 8, 10

1 Month
- Yolk (vitelline) sac
- Penduncle (early umbilical)
- Amniotic cavity
- Amnion
- Chorionic Villus
- Embryoinc Blood Vessels