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Microbiology lab > Lab Practical 1 > Flashcards

Lab Practical 1 Flashcards

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Biology 101 flashcards
1
Q

Brightfield microscope

A

allows light rays to pass directly through the eye

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2
Q

stage

A

horizontal platform that supports the microscope slide

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3
Q

light source

A

provides light through a voltage control

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4
Q

neutral density

A

reduces light intensity

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5
Q

ocular

A

two lenses to see through

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6
Q

objectives

A

name of the different strengths

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7
Q

what are the three objectives

A

low power high dry oil immersion

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8
Q

condenser

A

directs light to the slide

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9
Q

resolving power

A

ability to separate two objects

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10
Q

what can maximize resolving power?

A

blue filter immersion oil

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11
Q

what does bacteria’s cell wall made of?

A

peptidoglycan

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12
Q

what are the three shapes of bacteria

A

rod - bacilli spherical- cocci spirals- curved rods

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13
Q

are cocci motile?

A

no

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14
Q
A

cocobacillus

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15
Q
A

Bacillus

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16
Q
A

Fusiform

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17
Q

what are axial filaments

A

what allows spriocheates to be motile

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18
Q

what is aseptic technique

A

ensures there are no contaminants

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19
Q

what is a pure culture

A

isolating one bacteria from many

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20
Q

what are the two procdures for a pure culture

A

pour plate and streak plate

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21
Q

what are the types of streaking

A

quadrant

continuous

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22
Q
A

staphylococci

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23
Q

what is an example of a differential stain

A

gram stain

24
Q

what colour is gram positve

A

purple

25
Q

what colour is gram negative

A

pink

26
Q

what is a primary stain

A

crystal violet

27
Q

why are gram postive pruple?

A

thick walls of peptodoglycan

28
Q

what is the mordant in the gram stain?

A

iodine

29
Q

what does iodine do in a gram stain?

A

forms an insoluble complex in gram positive

30
Q

how is iodine removed in gram negative cells?

A

alchohol

31
Q

what is the decolorizer in gram negative

A

alcohol or acetone solution

32
Q

what is the counterstain

A

safranin

33
Q

what does safran DO

A

attached to lipid layer

34
Q

what are the two methods of spore staining

A

dorner and shaeffer fulton method

35
Q
A

simple stain

36
Q
A

capsule stain

37
Q
A

endospore stain

38
Q
A

gram stain

39
Q
A

acid fast

40
Q
A

gram positive pruple

gram negative pink

41
Q

what is the shaefer fulton method of spore staining

A

uses malachite green to stain endospore and safranin to stain vegetation

42
Q

what species to endospore belong to?

A

bacillus or clostridia

43
Q

what is the protein coat on an endosperm called

A

endosporium

44
Q

how can endospores be destroyed

A

autoclave

45
Q

what does the dorner method involve

A

Nigrosin is used to make a black endospore

46
Q

what is myolic acid

A

waxy material that coats cell

47
Q

what is the primary stain in acid fasting

A

carbolfuchsin

48
Q

what is the purpose of phenol in acid fast staining

A

allows carbolfuchsin to penetrate waxy coat

49
Q

what is the differerence between acid fast and non-acid fast

A

acid fast cannot be decolorized

non acid fast can be decolorized

50
Q

what must non-acid fast be counterstained with

A

methyl blue

51
Q

what colour are non acid fast cells

A

blue

52
Q

what colour are acid fast cells

A

pink

53
Q

what causes motion in becteria

A

flagella

54
Q

what is cheotaxis

A

move away from harmful bacteria

55
Q

what kind of rings do gram positive cells have

A

s and m

Microbiology lab (12 decks)

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