lab 39 and 40

Question 1

How do bacteria get nutrients?

Answer 1

by hydrolytic and degradative enzymes

Question 2

what do proteases degrade?

Answer 2

protein

Question 3

what does amylase and cellulose break down?

Answer 3

starch, cellulose

Question 4

How are starches then oxidized?

Answer 4

fermentation

Question 5

what are triglycerides broken down into?

Answer 5

fatty acids and glycerol

Question 6

what break down fats?

Answer 6

lipase

Question 7

what breaks down protein?

Answer 7

proteases

Question 8

why do bacteria digest material through exozymes?

Answer 8

because their rigid cell wall cannot go through phagocytosis

Question 9

what does tryptophanase do?

Answer 9

splits tryptophane into pyruvate and indole

Question 10

what does the hydrolyzation of urea bring about?

Answer 10

CO2 and ammonia leading to an alkaline ph

Question 11

how can you tell if an enzyme makes tryptophynase or not?

Answer 11

by indole production

Question 12

what can Proteus do ?

Answer 12

break down phenylalanine into an acid, which can be detected by ferric chloride

Question 13

why do bacteria expand all this energy?

Answer 13

to gain products for metabolism

Question 14

What does starch hydrolysis involve?

Answer 14

adding iodine to medium

if bacteria hydrolyzes starch then there will be clear zones

Question 15

what is casein hydrolysis

Answer 15

proteases in bacteria, will hydrolyze milk proteins such as casein into peptides and amino acids

Question 16

what is proteolysis?

Answer 16

turning milk proteins into peptides and amino acids

Question 17

how does tryptophan degradation work?

Answer 17

the degradation of tryptophan by tryptophanse can be detect by Kovac’s reagent. which forms a deep red color when indole is present

Question 18

what is a multiple test media/

Answer 18

one test that can show different results

Question 19

what does Kliger’s iron agar test for?

Answer 19

determines fermentation reactions for glucose and lactose

Question 20

what does SIM test for?

Answer 20

hydrogen sulfide, indole production and motility

Question 21

what does litmus milk test for?

Answer 21

fermentation, proteolysis

Question 22

what does the IMViC test for?

Answer 22

gram positive or negative

Question 23

what kind of bacteria is testes with kliger’s iron?

Answer 23

gram negative enteric bacteria

Question 24

what kind of bacteria does SIM test for?

Answer 24

determines if a substance is motile or not

Question 25

what does the litmus milk test for?

Answer 25

fermentation of lactose

Question 26

what does hydrogen sulfide test for?

Answer 26

degradation of protein