lab 39 and 40 Flashcards
How do bacteria get nutrients?
by hydrolytic and degradative enzymes
what do proteases degrade?
protein
what does amylase and cellulose break down?
starch, cellulose
How are starches then oxidized?
fermentation
what are triglycerides broken down into?
fatty acids and glycerol
what break down fats?
lipase
what breaks down protein?
proteases
why do bacteria digest material through exozymes?
because their rigid cell wall cannot go through phagocytosis
what does tryptophanase do?
splits tryptophane into pyruvate and indole
what does the hydrolyzation of urea bring about?
CO2 and ammonia leading to an alkaline ph
how can you tell if an enzyme makes tryptophynase or not?
by indole production
what can Proteus do ?
break down phenylalanine into an acid, which can be detected by ferric chloride
why do bacteria expand all this energy?
to gain products for metabolism
What does starch hydrolysis involve?
adding iodine to medium
if bacteria hydrolyzes starch then there will be clear zones
what is casein hydrolysis
proteases in bacteria, will hydrolyze milk proteins such as casein into peptides and amino acids
what is proteolysis?
turning milk proteins into peptides and amino acids
how does tryptophan degradation work?
the degradation of tryptophan by tryptophanse can be detect by Kovac’s reagent. which forms a deep red color when indole is present
what is a multiple test media/
one test that can show different results
what does Kliger’s iron agar test for?
determines fermentation reactions for glucose and lactose
what does SIM test for?
hydrogen sulfide, indole production and motility
what does litmus milk test for?
fermentation, proteolysis
what does the IMViC test for?
gram positive or negative
what kind of bacteria is testes with kliger’s iron?
gram negative enteric bacteria
what kind of bacteria does SIM test for?
determines if a substance is motile or not
what does the litmus milk test for?
fermentation of lactose
what does hydrogen sulfide test for?
degradation of protein
