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Microbiology lab > Lab final > Flashcards

Lab final Flashcards

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Biology 101 flashcards
1
Q

What are some of the most common types of bacteria?

A

bacillus, spherical (cocci), helical or curved

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2
Q
A

cococ bacilus

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3
Q
A

rod (bacilus)

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4
Q
A

staphylococci

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5
Q
A

streptococci

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6
Q

what are the common types of plating?

A

streak plate and pour plate

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7
Q

what is the primary stain in gram staining?

A

crystal violet

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8
Q

What is the mordant in graim staining?

A

iodine

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9
Q

what is the decolorizer in gram staining?

A

alcohol

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10
Q

what is the counter strain in gram staining?

A

safranin

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11
Q

what causes the staining in gram staining?

A

gram positive has a thicker wall of peptiloglycan

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12
Q

What result is this picture (gram stain)?

A

gram negative

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13
Q

what result is this gram stain?

A

gram positive

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14
Q

what species make endospores?

A

bacillus and clastridia

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15
Q

what is exosporium?

A

protective coating of endospore

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16
Q

where can endospores be made?

A

autoclave

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17
Q

what is mycolic acid?

A

protective coating on some bacteria like leprosy and tuberculosis

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18
Q

what is the primary stain in acid-fast?

A

carbolfuchsin

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19
Q

what do cells that are acid fast look like?

A

dark red

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20
Q

what colour is non-acid fast stain?

A

blue

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21
Q

what is motility determined by?

A

flagella

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22
Q

what is chemotaxis?

A

causes organism with flagella to move due to danger

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23
Q

what kind of rings does gram positive flagella have?

A

S and M rings

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24
Q

how can motility be determined?

A

by a wet mount test or hanging drop method

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25
Q

what is a psycrophile?

A

growth between -5 and 20.

supercool

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26
Q

what is a mesophile?

A

optimal growth between 50 and 20

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27
Q

what is a thermophile?

A

50 to 80

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28
Q

what is a hyperhermophile?

A
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29
Q

What is hypotonic?

A

outside is lower

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30
Q

what is hypertonic?

A

greater outside

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31
Q

what is a halotolerant organism?

A

tolerates salt like staph

32
Q

what is the difference between antimicrobials and antibiotics?

A

antibiotics kill or inhibit as well as antimicrobials

33
Q
A

filiform

34
Q
A

beaded

35
Q
A

echinulate

36
Q
A

effuse

37
Q
A

arborescent

38
Q
A

ring

39
Q
A

pellice

40
Q
A

flocculent

41
Q
A

f

42
Q
A
43
Q

what is a negative result of glucose?

A

green in both tubes

44
Q

what kind of result is this in glucose test?

A

fermentative and oxidative

45
Q

what is this result in a glucose test?

A

oxidative

46
Q

what results does this show in a durham sugar tube?

A

positive and negative

47
Q

what kind of results does this show for methyl red test?

A

positive and negative

48
Q

what is the VP test results shown?

A

positive and negative

49
Q

what does the methyl red test show?

A

shows mixed acid fermentation

50
Q

what does the durham sugar test show?

A

fermentation of sugars

51
Q

what does the VP test show?

A

ferment gucoe to make limited amounts of organic acids and butanadiol

52
Q

what does the citrate test show?

A

citrate as a carbon source

53
Q

is this a positive or nagtive citrate result?

A

positive blue

54
Q

what is the results seen for the oxidative test?

A

purpose positive

55
Q

what does the oxidative test show?

A

whether it can survive in an environment of oxygen

56
Q

what result is this in starch hydrolysis?

A

clear zone is positive for strach hydrolysis

57
Q

which is a positive result for casein hydrolysis?

A

clear zone on right indicates casein hydrolysis

58
Q

what does casein hydrolysis mean?

A

means that bacteria makes protealase

59
Q

which result if positive for fat hydrolysis?

A

dark blue pigmentation on left

60
Q

which of the following is positive tryptone hydrolysis?

A

the one wth the red ring at top

61
Q

what does tryptone degradation show?

A

that it degrades tryptophan to pyruvate

62
Q

what does a positive urease test look like?

A

pink tube

63
Q

what is the purpose of the urea test?

A

presence of urease

64
Q

what does kiglers iron agar do?

A

shows the fermenation of lactose and glucose

65
Q

what does this result show in kigler’s iron agar?

A

alkaline no change

66
Q

what does this result in kigler’s iron agar show?

A

hydrogren sulfide profuction

67
Q

what does this result in kigler’s iron agar show?

A

acid/acid with gas

68
Q

what does this result for kigler’s iron agar show?

A

alkaline and alkaline

69
Q

what does this result show in SIM medium?

A

hydrogen sulfide production

70
Q

what does this result show in SI medium?

A

indole production

71
Q

what are the results shown in this litmus milk test?

A

alkaline , acid, peptonization, coagulation

72
Q

what do the different litmus milk tests show?

A

alkaline- release of ammonia

acid- ferments milk sugar

peptonization- proteolytic that degrade protein

73
Q

what are the two types of hemolysis?

A

alpha and beta

74
Q

what does beta hemolysis look like?

A

clear zone with no red blood cells

75
Q

what color is a positive bile esculin test?

A

black

Microbiology lab (12 decks)

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