Lab Final Flashcards

1
Q

A new patient reports a history of morning stiffness in wrists, fingers and ankles bilateral RF. a titer is performed - the result o the titer is 1:60. your diagnosis or conclusion is:

A. Negative for RA but possible inflammatory condition. Additional tests needed
B. Positive for RA and patient should recover immediately follow adjustment
C. postiver for RA
D. Negative for RA and patient should recover immediately following adjustment.

A

A. Negative for RA but possible inflammatory condition. Additional tests needed

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2
Q

As mentioned in the virtual lab, which region of DNA is used in the identification of….

A. Intron
B. Conserved
C. Variable
D. Extron

A

C. Variable

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3
Q

identify th gram nature, cell shape, and cellular arrangement of the two species in the mixed smear micrograph (2).
(The rod shaped cells are pink in color and the spherical cells are purple here)

A. Gram negative staphylococcus
B. Gram positive streptobacilli
C. Gram positive singular bacilli
D. Gram negative streptobacilli
E. Gram positive staphylococcus
F. Gram negative singular bacilli

A

E. Gram positive staphylococcus
F. Gram negative singular bacilli

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4
Q

*This micrograph shows a gram stained smear with SPORES. Name the genus that is showing spores here (Also found in the lecture w/ picture)
A. Bacillus
B. Clostridium

A

B. Clostridium

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5
Q

P. Aeruginosa is a gram negative bacillus. Upon observation of a gram stained smear, multiple purple colored bacilli are observed. Which of the following is most likely?
A. Only the safranin step was omitted
B. The gram stain was performed correctly
C. Only the alcohol-acetone step was omitted
D. Only the iodine step was omitted

A

C. Only the alcohol-acetone step was omitted

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6
Q

The concentration of the antibiotic at the outer edge of the zone of inhibition is ____ …concentration closer to the disc.
A. The same
B. Lower
C. Higher

A

B. Lower

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7
Q

*3% KOH was used to test an unknown bacterium. Based on the image of that test, what conclusion can you make?
A. The species does not produce coagulase
B. This is a Gram positive species
C. This is a Gram negative species
D. This species produces coagulase

A

C. This is a Gram negative species

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8
Q

In the human immune system, which of the following are considered part of the innate immune system?
A. Plasma cells
B. Cytotoxic T-Cells
C. TH1 cells
D. CD8 cells
E. Dendritic cells

A

E. Dendritic cells

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9
Q

A person exposed for the second time to a pathogen years after first exposure, usually does not show any apparent symptoms of the disease because …
A. There are antigens from the first exposure present
B. Over time pathogens usually mutate into less virulent strains
C. There are plenty of antibodies from the first exposure present
D. After the first exposure the immune system is capable of converting the pathogen into a non-virulent strain
E. —due to naturally acquired immunity—there are specific antibody-producing cells present ready to trigger a rapid response against the pathogen

A

E. —due to naturally acquired immunity—there are specific antibody-producing cells present ready to trigger a rapid response against the pathogen

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10
Q

Essay: Name TWO of the consequences of the formation of anti-body-antigen complex, for each outline the path of one of the outcomes of the immu…not have to contain all the steps in the process map)
-Elimination of intracellular virus
-Elimination of extracellular virus
-Immunologic memory
-Ineffective virus (not able to infect host cells)

A
  1. Neutralization: Binding antibodies to bacteria and viruses has protective effects. It prevents interactions of microbial agents limiting their effectiveness of spreading to other toxins.
  2. Opsinization: Tags foreign antigens for elimination via phagocytosis
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11
Q

Reduced virulence is achieved by ______
A. Attenuation
B. Adjuncts
C. Apprehension
D. Adjuvants
E. Attrition

A

A. Attenuation

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12
Q

Match the correct type of test…
A. Precipitation test
B. Agglutination test
C. Complement fixation test
D. Labeled antibody test
E. Neutralization test

  1. _____ ELISA
  2. _____ Blood typing
  3. _____ Immunofluorecence
A
  1. D
  2. B
  3. D
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13
Q

Match the following descriptions:
A. Fever
B. Cell-mediated immune response
C. Phagocytosis
D. Complement
E. Inflammatory Response

  1. A process involving many proteins which leads to the lysis of foreign cells, gram-negative bacteria and enveloped viruses
  2. Cells ingest particulate matter from their environment
  3. A systematic reaction to infection
  4. Series of reactions after tissue injury to remove or contain offending agents and repair damage
  5. Is an adaptive immune response
A
  1. D
  2. C
  3. A
  4. E
  5. B
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14
Q

Gram negative color

A

Pink/red

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15
Q

Gram positive color

A

Violet/purple

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16
Q

Urease Test

A

-Red: Negative
-Yellow: Positive
-Yellow w/ Bubble: Positive w/gas

17
Q

Indole’s Test (Kovac’s Reagent)

A

-Positive: Red
-Negative: Yellow

18
Q

Citrate Test

A

-Positive: Blue
-Negative: Green

19
Q

MIC (Minimal Inhibitory Concentration)

A

Beginning point of growth after inhibition

20
Q

MLC (Minimal Lethal Concentration)

A

-Ensure agent would not be able to grow again after it is diluted

21
Q

Phenol Coefficient

A

How effective a compound is relative to phenol