Lab Exam Flashcards

1
Q

Who is the father of modern histology?

A

Xavier Bichat

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2
Q

What is tissue doctrin

A

A principle by which it is the structure of the organ that leads to its function

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3
Q

What is most useful about paraffin sectioning?

A

It allows samples to be handled at room temp (other than frozen)

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4
Q

Hematoxylin stains..

A

Acidic structures, like nucleic acids, nuclei and RER BLUE

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5
Q

Eosin stains…

A

Basic structures, proteins and membranes PINK

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6
Q

Why is the posterior pituitary more pink than the anterior

A

Because it is mostly made of axon terminals, not nuclei, so it stains with eosin

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7
Q

What are acidophils that stain pink with H&E

A

Somatotrophs and lactotrophs

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8
Q

What are basophils that stain blue/purple with H&E

A

Corticotrophs, thyrotrophs and gonadotrophs

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9
Q

What is trabeculae

A

Connective tissue that extends inwards from capsule in thyroid

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10
Q

Follicular cells produce…

A

Thyroid hormone

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11
Q

Parafollicular cells produce…

A

Calcitonin

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12
Q

How do you identify a parafollicular cell

A

Large nucleated cell outside the regular boundary of the follicular cell

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13
Q

Where are the parathyroid glands located

A

On the four poles on the backside of the thyroid

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14
Q

What cells make up the parathyroid

A

Chief cells, oxyphil cells and adipose cells

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15
Q

What does parathyroid produce

A

Parathyroid hormone

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16
Q

How do you identify a oxyphil cell

A

Dark nuclei and strongly eosinophilic cytoplasm

17
Q

The adrenal medulla is more…

A

Neuronal (therefore stains more blue/purple)

18
Q

Cells of the zona glomerulosa secrete…

A

Aldosterone

19
Q

Cells of the zona fasciculata secrete…

A

Cortisol

20
Q

Cells of the zona reticularis secrete…

A

DHEA and androstenedione (precursors of testosterone)

21
Q

What cells are found in the adrenal medulla

A

Chromaffin cells

22
Q

What are chromaffin cells

A

Modified postganglionic sympathetic neurons

23
Q

What do chromaffin cells secrete

A

Catecholamine, epinephrine, norepinephrine

24
Q

What stain is used on the pancreas

A

Gomori’s stain

25
Q

Beta cells stain…

A

Blue

26
Q

Alpha cells stain…

A

Pink

27
Q

What is aldehyde fuchsin stain

A

It stains beta cells of islet dark purple, the exocrine pancreas remains reddish-orange