Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Microbiology > Lab Exam 3 > Flashcards

Lab Exam 3 Flashcards

You may prefer our related Brainscape-certified flashcards:
Biology 101 flashcards
1
Q

Scientific method steps

A 
question
research
hypothesis
experiment
analyze data
conclusion
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Controlled variable

A

variable held constant

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Independent variable

A

changed variable

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Dependent variable

A

measured variable

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Control treatment

A

part of the experiment that is kept constant, used to compare results of the changed variable experiments

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Family Enterobacteriaceae characteristics

A

contain pathogens and non-pathogens
gram negative rods or coccobacillus
inhabit the gut of humans and animals
lactose non-fermenters are usually pathogens
family members are oxidase negative, glucose fermenters and nitrate reducers

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

S. aureus characteristics

A

yellow
growth and acid on MSA
coagulase positive
seen in skin infections and fatal septicemia

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

S. epidermidis

A

white
growth but no acid on MSA
coagulase negative

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Staphylococcus characteristics

A

seen in clusters
normal flora of skin and nasal membranes
S. aureus is a major pathogen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Streptococcus characteristics

A

seen in pairs and chains

normal flora of the throat, nose, mouth and intestine

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Streptococcus major pathogens

A

S. pyogenes
S. pneumoniae
S. agalactiae
S. faecalis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

S. pyogenes

A

causes strep throat, can lead to scarlet fever

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

S. pneumoniae

A

causes pneumonia

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

S. agalactiae

A

causes pneumonia, endocardidis and meningitis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

S. faecalis

A

causes endocardidis and food poisoning

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Alpha hemolysis

A

produces greenish, brownish zone around colonies

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Beta hemolysis

A

completely lyses blood cells around colonies

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Gamma hemolysis

A

no hemolysis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Mannitol salt agar test

A

contains 7.5% salt (high)

selective for staph

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Oxidase test

A

cyochrome oxidase reduces O2 to H2O in the final step of electron transport
positive result- black/purple within 20 sec

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Catalase test

A

splits H2O2 to H2O and O2

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

Salt broth test

A

6.5% NaCl

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

Bile eschulin test

A

hydrolysis of eschulin results in blackened percipitate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

Presumptive test

A

water tested for lactose fermenting bacteria, if gas is seen, there are presumed to be coliforms in the sample

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Q

Confirmed test

A

EMB agar inoculated with positive presumptive test samples, sample will grow if it is G- which is a coliform characteristic

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
26
Q

Completed test

A

IMViC test is done on colony grown in confirmed test, inoculated onto a nutrient agar slant and Durham tube of lactose broth, if both are positive, it is a coliform (G- non spore forming rod)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
27
Q

Petrifilm results

A

blue colonies with a gas bubble are E.coli, red colonies with a gas bubble are coliforms

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
28
Q

Glucose, lactose and sucrose fermentation tests

A

glucose, lactose or sucrose fermenter produces acid and gas, media color change to yellow

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
29
Q

MRVP mixed acid fermentation test media

A

Media: MRVP

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
30
Q

MRVP butanediol fermentation media

A

Media: MRVP

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
31
Q

Catalase production test reagent

A

H2O2

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
32
Q

SIM media

A

TSI/KIA

33
Q

Citrate utilization media

A

Media: simmon citrate

34
Q

SIM sulfide production test

A

if stab is black, positive for H2S production

35
Q

SIM indole production test reagent

A

Reagent: Kovacs

36
Q

TSI test

A

Yellow, growth on slant and butt= facultative anerobe, fermenter, G-

37
Q

Urease test media

A

Media: urea slant

38
Q

Nitrate reduction test media

A

Media: nitrate broth

39
Q

Lancefield groups

A

differentiate streptococcus based on carbohydrate antigen

40
Q

Hemolysis

A

used to differentiate streptococcus

can be alpha, beta or gamma

41
Q

S. pyogenes Lancefield group

A

Lancefield group A

42
Q

S. pyogenes arrangement

A

chains

43
Q

S. pyogenes hemolysis

A

Beta

44
Q

S. pyogenes optochin sensitive

A

optochin sensitive -

45
Q

S. pyogenes esculin hydrolysis

A

esculin hydrolysis -

46
Q

S. pyogenes NaCl tolerance

A

NaCl tolerance -

47
Q

S. pneumoniae arrangement

A

arranged in pairs

48
Q

S. pneumoniae hemolysis

A

alpha

49
Q

S. pneumoniae bacitracin sensitive

A

bacitracin sensitive -

50
Q

S. pneumoniae optochin sensitive

A

optochin sensitive +

51
Q

S. pneumoniae esculin hydrolysis

A

esculin hydrolysis -

52
Q

S. pneumoniae NaCl tolerance

A

NaCl tolerance -

53
Q

S. agalactiae lancefield group

A

Lancefield group B

54
Q

S. agalactiae arrangement

A

pairs

55
Q

S. agalactiae hemolysis

A

Beta

56
Q

S. agalactiae bacitracin sensitive

A

bacitracin sensitive -

57
Q

S. agalactiae optochin sensitive

A

optochin sensitive -

58
Q

S. agalactiae esculin hydrolysis

A

esculin hydrolysis -

59
Q

S. agalactiae NaCl tolerance

A

NaCl tolerance -

60
Q

S. faecalis lancefield group

A

Lancefield group D

61
Q

S. faecalis arrangement

A

pairs

62
Q

S. faecalis hemolysis

A

alpha and gamma

63
Q

S. faecalis bacitracin sensitive

A

bacitracin sensitive -

64
Q

S. faecalis optochin sensitive

A

optochin sensitive -

65
Q

S. faecalis esculin hydrolysis

A

esculin hydrolysis +

66
Q

S. faecalis NaCl tolerance

A

NaCl tolerance +

67
Q

Mannitol salt agar test result

A

sugar mannitol and the pH indicator phenoyl red

positive result- if sugar is fermented to acid and pH <6.8, media becomes yellow

68
Q

Catalase test result

A

positive= bubbles

69
Q

Salt broth test result

A

Positive result- turbidity, indicates growth

70
Q

MRVP mixed acid fermentation test reagent

A

methyl red

71
Q

MRVP mixed acid fermentation test result

A

red color is positive for mixed acid fermentation

72
Q

MRVP butanediol fermentation reagent

A

Reagent: Burrits reagent A and B

73
Q

MRVP butanediol fermentation result

A

pink color is positive for acetone production

74
Q

Citrate utilization result

A

Blue color change is a positive citrate reaction

75
Q

SIM indole production test result

A

color change to red is positive for indole production

76
Q

Urease test result

A

pink color = hydrolyzes urea

77
Q

Nitrate reduction test reagents

A

Reagents: A, B and/or C

78
Q

Nitrate reduction test result

A

Red color after nitrate reagent added is positive for nitrate reduction

Microbiology (6 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions