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Special Clinical Microbiology > Lab Exam 2 > Flashcards

Lab Exam 2 Flashcards

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Biology 101 flashcards
1
Q

What stage of a protozoan (cyst or troph) is most likely to be seen in a liquid stool?

A

troph

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1
Q

What are Charcot-Leyden crystals?

A

artifacts found in stool specimens, spindle shaped structures

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2
Q

How soon after collection should a semi-solid or liquid stool be examined?

A

within 30 minutes of passage

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3
Q
A

Diphyllobothrium latum proglottid

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3
Q

Which genera of parasites can be detected and identified by examination of a peripheral blood smear?

A

Leishmania, Plasmodium, Trypanosoma, Loa, Brugia, and Babesia

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3
Q

What is the principle of the zinc-sulfate procedure commonly used for the concentration of a stool specimen?

A

floatation

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3
Q

Why is it useful to perform the direct examination of a stool specimen using iodine?

A

used to observe cyst of protozoa. Darkens nuclei of cyst of protozoa

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4
Q
A

Ascaris lumbricoides decorticated egg

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4
Q
A

Schistosoma japonicum

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4
Q
A

Taenia saginata proglottid

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4
Q
A

Trichuris trichiura

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5
Q
A

Entamoeba coli troph

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6
Q
A

Balantidium coli troph

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7
Q
A

Entamoeba histolytica cyst

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8
Q

Why is it useful to perform the direct examination of a stool specimen using saline?

A

used to view anything motil, especially helminth eggs

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9
Q
A

Diphyllobothrium latum egg

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10
Q
A

Hymenolepsis dimunata

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11
Q

How soon after collection should a formed stool be examined?

A

macro and microscopic must be done same day, time permitting

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12
Q
A

Diphyllobothrium latum scolex

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12
Q
A

Fasciola/Fasciolopsis

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12
Q

Name several specimens other than stools that might be examined for ova and parasites.

A

Duodenal aspirates, perianal swabs, sputum, urine, blood, tissue biopsy

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14
Q
A

Giardia lamblia cyst

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15
Q
A

Enterobius vermicularis

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15
Q

What stage of a protozoan (cyst or troph) is most likely to be seen in a formed stool?

A

cyst

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16
Q

If a formed stool cannot be examined on the day of collection, what steps should be taken?

A

put specimen in preservative within 30 minutes and refrigerate

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17
Q
A

Trichinella spiralis encysted larva

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18
Q
A

Chilomastix mesnili troph

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20
Q
A

Entamoeba coli cyst

22
Q
A

Trichomonas

23
Q
A

Paragonimus westermanii

24
Q

What does PVA stand for?

A

poly vinyl alcohol

26
Q
A

Endolimax nana troph

27
Q

What type of container is best to use for the collection and transporting of a stool specimen?

A

Clean and dry, does not need to be sterile

28
Q

What is the significance of Charcot-Leyden crystals?

A

indicates inflammation, parasitic infections, or allergic reactions

29
Q
A

Iodamoeba bütschlii troph

29
Q

Name several species of protozoa that have “emerged” as significant pathogens in recent years due to the spread of AIDS.

A

Pneumocystis carinii, Cryptosporidium, Toxoplasma gondii

30
Q

What are Charcot-Leyden crystals thought to be composed of?

A

ruptured eosinophil granules

32
Q
A

Dientamoeba fragilis

34
Q
A

Necator americanus (hookworm) rhabditiform larva

35
Q

What organism is the scotch tape preparation used for?

A

Enterobius vermicularis (pinworms)

36
Q
A

Trypanosoma

37
Q
A

Schistosoma mansoni

38
Q
A

Iodamoeba bütschlii cyst

38
Q
A

Clonorchis sinensis

39
Q
A

Ascaris lumbricoides unfertilized egg

39
Q
A

Strongyloides stercoralis rhabditiform larva

41
Q
A

Taenia solium scolex

42
Q

Name several artifacts that can be found in ova and parasite preparations.

A

plant fibers, pollen grains, meat fibers, epithelial cells, Charcot-Leyden crystals, and WBCs

43
Q

Name the two procedures commonly used for the concentration of a stool specimen?

A

Formalin-ethyl acetate and zinc-sulfate

44
Q
A

Ascaris lumbricoides fertilized egg

46
Q

What is the principle of the formalin-ethyl acetate procedure commonly used for teh concentration of a stool specimen?

A

sedimentation

47
Q
A

Taenia solium proglottid

49
Q
A

Hymenolepsis nana

51
Q
A

Balantidium coli cyst

52
Q
A

Hymenolepsis dimunata

54
Q
A

Chilomastix mesnili cyst

55
Q
A

Taenia saginata scolex

56
Q
A

Necator americanus (hookworm) egg

58
Q
A

Schistosoma haematobium

60
Q
A

Giardia lamblia troph

61
Q
A

Hymenolepsis nana

62
Q
A

Hymenolepsis dimunata

63
Q
A

Entamoeba histolytica troph

64
Q

What fixative should be used with a liquid stool?

A

PVA

65
Q
A

Taenia saginata egg

66
Q

What fixative should be used with a formed stool?

A

10% formalin

67
Q

What are teh four components of the procedure for the laboratory examination of a stool specimen for ova and parasites?

A

Macroscopic exam, direct microscopic exam, concentration, permanent stain smear

68
Q
A

Endolimax nana cyst

Special Clinical Microbiology (5 decks)

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