LAB - ELISA FINALS

Question 1

Competitive ELISA basic princ

Answer 1

Primary Ab (unlabeled) is inc w/ sample Ag

Ag-Ab complexes are added to 96-well plates wc are pre-coated w/ the same Ag

Unbound Ab is removed by washing the plate

Secondary Ab specific to the primary ab & conj w/ enzyme is added

Substrate is added & remaining enzymes elicit CHROMOGENIC/FLUORESCENT SIGNAL

Question 2

Competitive ELISA adv

Answer 2

High spec (2 abs used)
Suitable for complex samples (ag doesnt req purifxn)
Flex and sensi (direct and indi)