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CC1 LEC > Basic Principles and Boring Stuff > Flashcards

Basic Principles and Boring Stuff Flashcards

1
Q

Anatomic Pathology

A

Histopathology

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2
Q

Clinical Pathology 6

A 
Serology
Microbiology
Clinical Chemistry
Hematology
Clinical Microscopy
Blood Bank
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3
Q

Concerned with the analysis of biochemical byproducts in biological fluids

A

Clinical Chemistry Laboratory

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4
Q

Pure Blood Chemistry

A

Lipids, Carbohydrates and Protein

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5
Q

Enzymology

A

Enzymes

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6
Q

Endocrinology

A

Hormones

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7
Q

Toxicology

A

Drugs of abuse, Heavy metals, Poison, etc.

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8
Q

Units of Measure: Components of quantitative laboratory results 2

A

Number

Unit (based on the SI system)

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9
Q

Quantitative laboratory results

2

A

1 Substance concentration
e.g, moles
2 Mass of substance
e.g., mg/dL, g/dL, g/L, mEq/L, and IU

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10
Q

Reagents 3

A

Chemicals
Reference Materials
Water Specifications

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11
Q

Chemicals 5

A

Analytical Grade (AR)

Ultrapure Reagent

Chemically pure (CP)

United States Pharmacopeia (USP) and National Formulary (NF) Grade

Technical or commercial grade

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12
Q

Suitable for most analytic procedures

Carry designations as AR or ACS and For Laboratory Use or ACS standard-Grade Reference materials

A

ANALYTICAL GRADE (AR)

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13
Q

Suitable for techniques that require extremely pure chemicals (e.g. AAS, EIA, MDx)
Carry designations of HPLC or chromatographic

A

Ultrapure chemicals

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14
Q

Impurity limitations are not stated
Preparation is not uniform
Not recommended for clinical laboratories.

A

Chemically pure (CP)

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15
Q

Used to manufacture drugs

Purity standards are based on the criterion of not being injurious to man.

A

USP and NF Grade

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16
Q

Reference Materials 2

A

1Primary standard
Substance of exact known concentration and purity.

2Secondary standard
Substance of lower purity with concentration determined by comparison with a primary standard.

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17
Q

Substance of exact known concentration and purity.

A

Primary standard

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18
Q

Substance of lower purity with concentration determined by comparison with a primary standard.

A

Secondary standard

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19
Q

Water Specifications 5

A 
Distilled water
Deionized water
Reverse Osmosis (RO) water
Ultrafiltration and nanofiltered water
Reagent grade water
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20
Q

Obtained by initial filter, followed by RO, deionization and a 0.2 mm filter.

A

Reagent grade water

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21
Q

For test methods requiring minimum interference
trace metal analysis by FES and AAS
Gas, pH, enzyme and electrolyte analysis

A

Type I water

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22
Q

For analytical preparations

reagent, QC and standard preparation

A

Type II water

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23
Q

Glassware washing

A

Type III/autoclave wash water

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24
Q

Warming components is necessary for analytical procedures and is accomplished by circulating water/ice baths or heating/cooling metal blocks.

A

Thermometers/Temperature

25
Q

Thermometers/Temperature 3

A

Liquid-in-glass
Electronic thermometer or thermistor probe
Digital thermometer

26
Q

Use of a colored liquid or mercury encased in plastic/glass material with a bulb at one end a graduated stem

A

Liquid-in-glass

Partial Immersion (with bulb)
Total Immersion
Surface thermometer – for incubators or heating oven

27
Q

Fast reading with millisecond response time

A

Electronic thermometer or thermistor probe

28
Q

Glassware 6

A 
Kimax® /Pyrex® (borosilicate)
Corex® (aluminosilicate)
High silica
Vycor® (acid or alkali resistant)
Low actinic (amber colored)
Flint  glass (lime soda)
29
Q

Plastic ware – are usually disposable lab supplies

6 Example of commonly used resins:

A 
Polystyrene
Polyethylene
Polypropylene
Tygon®
Telon®
30
Q

Laboratory vessels 3 3

A

Pipets
Burets
Syringes

Class A volumetric flask
Erlenmeyer flasks and Griffin beaker
Graduated cylinder

31
Q

Calibrated to hold one exact of liquid (TC)

A

Class A volumetric flask

32
Q

Hold different volume

Used in reagent preparation

A

Erlenmeyer flasks and Griffin beaker

33
Q

Used to measure volumes of liquid

A

Graduated Cylinder

34
Q

Glass or plastic utensils used to transfer liquids

A

Pipets

35
Q

Classification of Pipet According to Design or Calibration Marks
2

A 
To contain (TC)
To deliver (TD)
36
Q

Used for viscous samples
Uses mercury as calibrating medium
Proper use requires rinsing of the pipet with the final solution after content are delivered into the diluent (rinsing technique)

A

To contain (TC)

37
Q

Used for non-viscous samples

Uses distilled water as calibrating medium

A

To Deliver (TD)

38
Q

with etched ring or two small, close continuous ring. The last drop of fluid need to be blown

A

Blowout pipet

39
Q

without marking. Drains completely.

A

Self-draining

40
Q

Classification of Pipet According to Use 4

A

Measuring or graduated pipets
Volumetric or Transfer pipet
Mechanical or Automatic pipet
Micro pipet

41
Q

Graduated uniformly along its length

Designed to deliver any amount within its capacity

A

Measuring or graduated pipets

42
Q

Has graduation marks to the tip

Generally a blowout pipet

A

Serolic pipet

43
Q

No graduation marks to the tip

Self draining

A

Mohr Pipet

44
Q

Designed to transfer one volume

A

Transfer pipet

45
Q

For viscous fluids (blow out pipet)

A

Ostwald-Folin pipet

46
Q

For aqueous solutions (self draining)

A

Volumetric pipet

47
Q

No calibration marks

For transferring fluids without consideration of a specific volume

A

Pasteur pipets

48
Q

Automatic pipets 2

A

Micropet – deliver amount 1ml

Macropipet – deliver amount >1ml

49
Q

The piston moves in the tip and comes in contact with the liquid

A

Positive displacement

50
Q

The piston does not come in contact the liquid

A

Air displacement

51
Q

Used to transfer small volumes in blood gas analysis, chromatography or electrophoresis

A

Syringes

52
Q

Uses hygroscopic substance that take up water/moisture on exposure to air.

A

Desiccators and Desiccants

53
Q

Balances

2

A

Electronic Top-loading balance

Analytical Balance

54
Q

used for knowing the mass of the test sample with greater quantity.
Used for preparative experiments

A

Electronic Top-loading balance

55
Q

for preparation of primary standards
With sliding transparent doors
Measure exact mass but with lower capacities (operating ranges 0.01 mg to 160 g)

A

Analytical balance

56
Q

a process in which a centrifugal force is used to separate solid matter from a liquid suspension.

Consist of head/rotor (attached to the shaft of the motor), carrier and shields.

A

Centrifugation

57
Q

The speed/centrifugal force is expressed by:

2

A

Revolution per minute (RPM)

Relative centrifugal force (RCF) or gravities (g)

58
Q

Paper, cellulose, polyester fibers and column materials

A

Filtration

CC1 LEC (10 decks)

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