Lab E Flashcards
What is the special property of Taq polymerase that allows it to work in PCR?
It is heat resistant WOOOO~
Once you have loaded your sample on the plate, you can dispose of the tube containing your cheek cells by
putting it in the regular trash
T/F: In the DNA isolation lab, you must wear gloves in order to prevent any of your cells from contaminating other students’ samples
true
T/F: Mitochondrial DNA can be inherited from either an individual’s father or mother.
false (only mother)
The technique used for DNA amplification is
PCR
Which of the following is not required for PCR?
- buffer solution
- dNTPs
- Taq polymerase
- sequencing primer
- two primers that bind to denatured genomic dna at complementary sequences
sequencing primer
Isolated DNA is susceptible to enzymes that degrade DNAses. They require the cofactor ________ which the chelex solution binds to tightly.
Mg 2+
According to the LS Core Lab Safety Sheet for this lab, which of the following is a potential hazard you will face in the DNA Isolation lab?
chemical causing eye irritation
T/F: You can remove your lab coat once you are done with the wet lab portion of the DNA Isolation experiment, even if other people in the room are still working with Chelex.
False
Mitochondrial DNA is used to follow maternal inheritance and sequences on the Y chromosome is used to follow paternal inheritance. Why is it not practical to analyze paternal inheritance in this class?
Because only men have a Y chromosome, the women wouldn’t be able to participate.
Which of these descriptions of PCR is not correct?
- PCR stands for polymerase chain reaction
- PCR required a single primer
- in the first step of PCR, DNA parental strands are denatured at 94 degrees Celsius
- Taq polymerase is used because it is resistant to heat denaturation
- PCR is used to make more copies of DNA for sequencing
PCR required a single primer
The purpose of Chelex in this lab is to (select all that apply):
- Bind to the Mg2+ in the DNA extract
- Prevent the DNA from being degraded
In this lab, DNA will be collected from:
cheek cells/saliva
PCR
amplifies specific DNA sequence
T/F: mtDNA is more abundant per cell than nuclear DNA
true
