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MCB > Lab culture > Flashcards

Lab culture Flashcards

1
Q

Designed to support the growth of microorganisms or cells
S must contain the right nutrients, sufficient
moisture, a properly adjusted pH and a suitable oxygen level or no
oxygen
It also must be initially sterile and be incubated at the proper temperature

A

Culture media

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2
Q

Contains the necessary nutrients to promote microbe
cultivation
Specially designed
S Promote growth of fastidious organisms
S Help in the isolation, characterization, and identification of
microbes
S Eliminate contaminating microbes

A

Microbiological Culture Media

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3
Q

– microbes introduced to culture medium to initiate
growth

A

Inoculum

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4
Q

– microbes that multiply and grow in or on a culture
medium

A

Culture

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5
Q

– a complex polysaccharide derived from marine alga,
liquifies at ___°C and stays liquid at sea level until the temp. drops at
40°C

A

Agar; 100

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6
Q

Physical State of media?

A

Liquid Media
Solid Media
Semi-Solid Media

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7
Q

Consist of the growth nutrients
dissolved in water
Do not contain agar
Also known as _____

A

Liquid culture media
broths or
infusions

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8
Q

Solidifying agent (agar) added to
liquid media
Provides firm substrate that
allows the formation of colonies
on the surface or within
Contains ____ % agar

A

Solid media
1.5 – 1.7

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9
Q

Example of Solid media?

A

Nutrient Agar, Blood Agar

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10
Q

Small proportion of solidifying
agent to maintain a thick
consistency without solidifying at
room temperature
Used to allow motility of the
organism under study
Contains____% agar

A

semi-solid media
0.5-0.7

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11
Q

Example of Semi-Solid media?

A

Sulfur Indole Motilty media
(SIM), Motility test medium

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12
Q

Two Chemical Composition

A

Defined or Synthetic
Complex or Non-synthetic

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13
Q

Precise chemical composition
and amount of all components
are known

A

Defined or Synthetic Media

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14
Q

Used when trying to determine
an organism’s specific growth
requirements

A

Defined or Synthetic Medie

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15
Q

Contain nutrients in which the
exact components or their quantity
is not completely known

A

Complex or Non-synthetic
Media

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16
Q

May come from animal, plant,
or yeast extracts
Most of the organisms used will
grow in these nutrient rich
conditions

A

Complex or Non-synthetic
Media

17
Q

Functional Class of media? (6)

A

General Purpose Media
Minimal Media
Selective Media
Differential Media
Transport Media
Enriched Media

18
Q

Promote the growth of a wide spectrum of microbes, nonpathogenic and pathogenic, bacteria and fungi alike

A

General Purpose Media

19
Q

Nutrient Agar or Broth, Trypticase Soy Agar or Broth

A

General Purpose Media

20
Q

Contains the minimum nutrients possible for colony growth,
generally without the presence of amino acid
-Can differentiate against mutants
-Typically contains a carbon source for bacterial growth
(glucose, succinate)

A

Minimal Media

21
Q

Minimal media example

A

M9 Minimal Medium

22
Q

Contain ingredients to inhibit the growth of certain microbes in a mixture while allowing the growth of others
May contain extra salt (NaCl), dye, or antibiotic to inhibit the growth of intolerant or sensitive organisms

A

Selective Media

23
Q

Example of selective

A

Eosin Methylene Blue Agar, Saboraud’s Dextrose Agar, Hektoen Enteric Agar

24
Q

Can distinguish one microorganism type from another
growing on the same culture plate
Contain specific chemicals to indicate which species possess
and which lack a particular biochemical process

A

Differential Media

25
Q

Examples of differential

A

Mannitol Salt Agar, MacConkey Agar, Salmonella-Shigella
Agar

26
Q

Contain nutrients required to support
the growth of a wide variety of
organisms
Addition of supplements and growth
factors (vitamins or amino acids) allow
the growth even of fastidious organisms

A

Enriched Media

27
Q

having special nutritional
requirements, requires many growth factors

A

Fastidious:

28
Q

Examples of enriched

A

Blood Agar, Chocolate Agar

29
Q

Temporary storage media of specimens
being transported to the laboratory for
cultivation
-Maintain the viability of all organisms
in the specimen without altering the
concentration
-Typically contain only ______
-Lack of carbon, nitrogen and organic
growth factors prevents microbial
multiplication

A

Transport Media
buffers and salt

30
Q

Plates __mL
Slants __ mL
Broths ___ mL
Stabs or Deeps ___ mL

A

20
8
10
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MCB (6 decks)

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