Lab culture Flashcards
Designed to support the growth of microorganisms or cells
S must contain the right nutrients, sufficient
moisture, a properly adjusted pH and a suitable oxygen level or no
oxygen
It also must be initially sterile and be incubated at the proper temperature
Culture media
Contains the necessary nutrients to promote microbe
cultivation
Specially designed
S Promote growth of fastidious organisms
S Help in the isolation, characterization, and identification of
microbes
S Eliminate contaminating microbes
Microbiological Culture Media
– microbes introduced to culture medium to initiate
growth
Inoculum
– microbes that multiply and grow in or on a culture
medium
Culture
– a complex polysaccharide derived from marine alga,
liquifies at ___°C and stays liquid at sea level until the temp. drops at
40°C
Agar; 100
Physical State of media?
Liquid Media
Solid Media
Semi-Solid Media
Consist of the growth nutrients
dissolved in water
Do not contain agar
Also known as _____
Liquid culture media
broths or
infusions
Solidifying agent (agar) added to
liquid media
Provides firm substrate that
allows the formation of colonies
on the surface or within
Contains ____ % agar
Solid media
1.5 – 1.7
Example of Solid media?
Nutrient Agar, Blood Agar
Small proportion of solidifying
agent to maintain a thick
consistency without solidifying at
room temperature
Used to allow motility of the
organism under study
Contains____% agar
semi-solid media
0.5-0.7
Example of Semi-Solid media?
Sulfur Indole Motilty media
(SIM), Motility test medium
Two Chemical Composition
Defined or Synthetic
Complex or Non-synthetic
Precise chemical composition
and amount of all components
are known
Defined or Synthetic Media
Used when trying to determine
an organism’s specific growth
requirements
Defined or Synthetic Medie
Contain nutrients in which the
exact components or their quantity
is not completely known
Complex or Non-synthetic
Media
May come from animal, plant,
or yeast extracts
Most of the organisms used will
grow in these nutrient rich
conditions
Complex or Non-synthetic
Media
Functional Class of media? (6)
General Purpose Media
Minimal Media
Selective Media
Differential Media
Transport Media
Enriched Media
Promote the growth of a wide spectrum of microbes, nonpathogenic and pathogenic, bacteria and fungi alike
General Purpose Media
Nutrient Agar or Broth, Trypticase Soy Agar or Broth
General Purpose Media
Contains the minimum nutrients possible for colony growth,
generally without the presence of amino acid
-Can differentiate against mutants
-Typically contains a carbon source for bacterial growth
(glucose, succinate)
Minimal Media
Minimal media example
M9 Minimal Medium
Contain ingredients to inhibit the growth of certain microbes in a mixture while allowing the growth of others
May contain extra salt (NaCl), dye, or antibiotic to inhibit the growth of intolerant or sensitive organisms
Selective Media
Example of selective
Eosin Methylene Blue Agar, Saboraud’s Dextrose Agar, Hektoen Enteric Agar
Can distinguish one microorganism type from another
growing on the same culture plate
Contain specific chemicals to indicate which species possess
and which lack a particular biochemical process
Differential Media
