[LAB] ACTIVITY 4: ROUTINE URINALYSIS: CHEMICAL EXAMINATION Flashcards
pH PRINCIPLE
Double-indicator system
pH REAGENTS
Methyl red, bromthymol blue
Protein REAGENTS
Multistix: tetrabromphenol blue
Chemstrip: 3’,3”,5’,5”-tertachlorophenol 3,4,5,6-tetrabromsulfonphthalein
Protein PRINCIPLE
Protein error of indicators
Glucose PRINCIPLE
Double sequential
enzyme reaction
Glucose REAGENTS
Multistix: glucose oxidase,
peroxidase, potassium iodide
Chemstrip: glucose oxidase,
peroxidase, tetramethylbenzidine
Ketones PRINCIPLE
Sodium
nitroprusside
reaction
Ketones REAGENTS
Sodium nitroprusside, glycine
(Chemstrip)
Blood PRINCIPLE
Pseudoperoxidase
activity of Hb
Blood REAGENTS
Mulstix: diisopropylbenzene
dehydroperoxide
tetramethylbenzidine
Chemstrip:
dimethyldihydroperoxyhexane
tetramethylbenzidine
Bilirubin PRINCIPLE
Diazo reactions
Bilirubin REAGENTS
Multistix: 2,4-dichloroaniline
diazonium salt
Chemstrip: 2,6-dichlorobenzene
diazonium salt
Urobilinogen PRINCIPLE
Multistix:
Ehrlich’s aldehyde
reaction
Chemstrip: diazo
reaction
Urobilinogen REAGENTS
Multistix: p-
dimethylaminobenzaldehyde
Chemstrip: 4-methoxybenzene-
diazonium-tetrafluoroborate
Nitrite PRINCIPLE
Greiss reaction
Nitrite REAGENTS
Multistix: p-arsamilic acid,
tetrahydrobenzo(h)-quinolin-3-ol
Chemstrip: sulfanilamide,
hydroxytetrahydro benzoquinoline