Lab 9-10 Flashcards
LAB9. Differential stain
Dyes different kinds of bacteria in contrasting colors
LAB9 What do you call it when bacterias have a shape of a square?
Tetrads
LAB9. Cube shaped bacteria are called..?
Sarcinae
LAB9. bacillus vs. Bacillus(italics)
bacillus=shape
Bacillus(italics)= specific type of microbe(genome name)
LAB9. Simple stain is defined as
A procedure that stains all cells the same color
LAB10. Mordant causes
A stain to become more tightly bound to the cell
LAB10. What specific stain is caused by mordant?
Grams iodine (orange)does this by intensifying the ionic chemical bond between crystal violet and the bacteria -still stain purple
LAB10. Decolorizer is the
Differentiating step of the gram stain = Ethyl alcohol
LAB10. Ethyl alcohol removes..
The stain from some bacteria, such as gram-negative, which turns out to become colorless
LAB10. Which type of bacteria is not decolorized ethyl alcohol?
Gram-positive bacteria, therefore remain purple
LAB10. Gram-positive bacteria stain…?
Purple
LAB10. Gram-negative bacteria stain..?
Red or pink
LAB10. The permeability theory
This theory proposes that bacteria stain differently because of chemical and structural differences in their cell walls.
LAB10. Old cultures
The permeability theory can also explain why gram-positive bacteria sometimes come out red and set a purple due to the Broken down cell wall
LAB10. Which will retain their stain more viruses or bacteria?
Bacteria because they are larger than viruses and therefore they will retain the stain more due to their size and structure.
