Lab 20,22,23

Question 1

LAB22. What is considered to be a lawn?

Answer 1

When a pathogen is spread all over the surface of the agar

Question 2

LAB22. The classic technique for determining which antibiotics will be effective against a particular micro organism is the…?

Answer 2

Kirby Bauer sensitivity test

Question 3

LAB22. If the bacterium is sensitive to a particular antibiotic what will happen to the growth?

Answer 3

Growth will be inhibited and a clear zone will be seen around the antibiotic disc.

Question 4

LAB20. List two medical examples of how old true violet light is used to control microorganisms

Answer 4

1. ) The medical industry uses ultraviolet light to sterilize rooms & equipment so pathogens won’t replicate
2. ) medical treatment, such as cancer, so pathogens don’t replicate as much

Question 5

LAB22. Define PPNG

Answer 5

Penicillinase Producing Neisseria Gonorrhea

• A special type of gonorrhea that cannot be cured by penicillin but can be cured by other antibiotics

Question 6

LAB22. Define MIC

Answer 6

The lowest concentration of drug that stops the growth of microorganisms

Question 7

LAB22. Define superinfection

Answer 7

Infection occurring after or on top of an earlier infection

especially following treatment with broad-spectrum antibiotics

Question 8

LAB23. Lysozyme dissolves the chemical bond between…

Answer 8

NAG and NAM within the backbone of the peptidoglycan molecule

Question 9

LAB23. By breaking down the cell wall Peptidoglycan, lysozyme causes the bacteria to…?

Answer 9

Lyse

-especially gram-positive bacteria

Question 10

LAB23. Lysozyme is blocked from what type of bacteria?

Answer 10

Gram-negative, due to its outer cell wall

Question 11

LAB23. If Micrococcus luteus is a gram-positive bacteria, will lysozyme be able to enter and break the cell wall?

Answer 11

Yes

Question 12

Lab23. If Serratia marcescens is a gram-negative bacteria will license and be able to enter and break the cell wall?

Answer 12

No