Lab 13,14&19

Question 1

LAB13. Myobacterium is different from other bacteria because it has a layer of..?

Answer 1

Mycolic acid

Question 2

LAB13. Mycolic acid is A waxy material that causes cells to..?

Answer 2

Stick together to form raised in wrinkled colonies and prevents aqueous chemicals to penetrate the waxy cell wall

Question 3

LAB13. What is the result of the Gram stain on Mycobacterium?

Answer 3

Gram-positive

Question 4

LAB13. Name two species of micro organisms that are acid fast and the disease they cause.

Answer 4

Mycobacterium tuberculosis = Tuberculosis

Mycobacterium leprae= Leprosy

Question 5

LAB13. What would bacillus look like with an acid-fast stain?

Answer 5

It will absorb the methylene blue instead.
Because it is not an acid-fast bacteria, it will not retain the red from Carbil fusion and it would easily be discolorized

Question 6

LAB13. What specimen from a tuberculous is patient would you collect to perform an acid-fast stain?

Answer 6

Phlegm or septum

Question 7

LAB14. What are the three methods for bacteria to recombine genes?

Answer 7

Transformation, transduction, and conjugation

Question 8

LAB14. What is Genetic recombination?

Answer 8

Allows bacteria to acquire combinations of antibiotic resistant genes

Question 9

LAB14. Bacteria do not reproduce sexually but instead multiply through simple….?

Answer 9

Binary fission

Question 10

LAB19. Heat can be utilized in the form of…?

Answer 10

Moist heat
(boiling water, steam under pressure)

Or dry heat
(dry oven, incineration)

Question 11

LAB19. Thermal Death Point (TDP)

Answer 11

Is the temperature at which an organism is killed in 10 minutes

Question 12

LAB19. Thermal Death Time (TDT)

Answer 12

Is the time required to kill a suspension of cells or spores at a given temperature

Question 13

LAB19. Dray he kills organisms through a process of..?

Answer 13

Protein oxidation

Question 14

LAB19. Moist heat is more effective and kills microorganisms by…?

Answer 14

Denaturing proteins