LAB 11-12 Flashcards
LAB11. What types of substances can a capsule be composed of?
Polysaccharides, proteins or both
LAB11. Capsules inhibit..?
Phagocytosis
-by our neutraphils & macrophages
LAB11. Since the capsular material does not stain well, what is the first procedure of staining a capsule ? Second?
1st. Staining the background of the slide =negative stain
2nd. A stain is applied that colors the cytoplasm of the bacteria.
The capsule should result in a “light halo”
LAB11. What effect does the presence of a capsule have on pathogenicity?
Ability for an organism to cause disease/infection. No phagocytosis can be successfully done.
LAB11. Opsonin means
Any molecule that enhances phagocytosis by marking an antigen for an immune response
LAB11. Define negative stain
Used to stain the background of the slide.
Background =dark
Bacteria=clear
LAB11. Capsule
A capsule has a thick layer of substance found on the surface of some species of bacteria.
LAB12. Which two bacteria produce spores?
Bacillus and clostridium
LAB12. When this board is first produced inside the cell it is called..?
An endospore
LAB12. If an endospore can develop in the middle of the rod it is called..?
Central spore
LAB12. If an endospore is at the end of the rod it is called..?
Terminal spore
LAB12. When is it considered when a spore is free and is in a dormant resistant stage?
When the cytoplasm around the endospore disintegrates
LAB12. Can typical stain procedures such as Gram stain penetrate through a spore wall?
No.
LAB12. Are spores involved in bacterial reproduction?
Yes, they transfer genetic material from donor cell to the recipient cell= recombination
LAB12. Endospores are produced by
Prokaryotes
