Lab 7

Question 1

Hypertonic

Answer 1

h20 goes out of the blood cell

Question 2

isotonic

Answer 2

equal amounts of h20 goes in and out

Question 3

Hypotonic

Answer 3

h20 goes into blood cell

Question 4

Blood can be separated by____and differential centrifugation to produce three protein-containing fractions which are___

Answer 4

osmotic lysis

plasma,lysate(red blood cell cytoplasm), and cell membrane

Question 5

The lystae fraction we plan to isolate in the lab is found in the plasma- true or false

Answer 5

false
lysate in found in the erythrocytes after putting in a hypotonic solution causing it to burst and removing plasma and centrifugation more, the cell e membrane

Question 6

Proteins easily degrade when handled in a laboratory setting due to ___celluar protease released from cellular compartments following osmotic lysis.

Answer 6

ubiquitous

Question 7

By keeping samples on ice enzymatic degradation by __is slowed

Answer 7

proteases

Question 8

Protein samples are stored at

Answer 8

-20 to -80

Question 9

Protein concentrations of a sample can be measured using the

Answer 9

Bradford assay

Question 10

Explain Bradford assay

Answer 10

the acidified component coomassie brilliant blue binds and reacts to arginine and to aromatic acids like tyrosine, phenylalanine and tryptophane. It causes a change in wavelength

Question 11

Known concentrations of ___are commonly used for a protein standard

Answer 11

bovine serum albumin(BSA)

Question 12

Coomassie brilliant dye shifts wavelength from___to___

Answer 12

470nm to 595nm