Lab 2

Question 1

How to do Koehler illumination?

Question 2

Steps for Koeher Illumination

Answer 2

Close field iris unit small circle
center the circle of light by screws
move condenser knob till sharp edges
open field iris till light just disappears from view

Question 3

A fluoeochrome—

Answer 3

absords light at a lower wavelength and emits light at a higher wavelength

Question 4

DAPI stain binds to

Answer 4

A-T rich regions of DNA

Question 5

Binds to F-actin, a component of the cytoskeleton

Answer 5

Phalloidin-Rhodamine stain

Question 6

paraformaldehyde

Answer 6

cells are treated with PFA to fix the cells and permeabilizxe the membrane which allows dyes to enter cells

Question 7

Convert whats 100 nm to micrometer(um)

Answer 7

0.1 um. divid by 1000

Question 8

What aprt of the human cell will be visible when using DAPI

Answer 8

nucleus

Question 9

Rodamine is cinjugated ti phalloidin. Which of these has fluoresent properties

Answer 9

rhodamine

Question 10

IN ZOE Fluoresent where do u focus the cells?

Answer 10

In live mode only

Question 11

There are three coloujr channels in fluorescent mircoscope, which do DAPI and rhodamine-phalloidin use?

Answer 11

Blue with DAPI
Red with rhodamine-phalloidin

Question 12

Depth of field the high power lenses have a —-depth of focus than low power ones

Answer 12

shorter

Question 13

High power lenses have a __ working distance than lower power ones

Answer 13

shorter

Question 14

High power lenses have a ___field diameter than low power lense

Answer 14

smaller