Lab 6 Flashcards

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1
Q

What is the optimal temp for S.cerevisiae

Doubling is

A

30C
1.8 hours

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2
Q

Optimal temp for E.coli

Doubling

A

0.33 hours

37C

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3
Q

HeLa cells optimal temp is____Agitation is___Doubling is___

A

37C
mild
23 hours

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4
Q

What happened in this experiment

A

testing proficiency of CPA’s(glycerol, DMSO)

thaw cells and wash off CPA-centrifugation
stain cells with methylene blue
use hemocytometer to count cells

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