Lab 6 Flashcards
1
Q
What is the optimal temp for S.cerevisiae
Doubling is
A
30C
1.8 hours
2
Q
Optimal temp for E.coli
Doubling
A
0.33 hours
37C
3
Q
HeLa cells optimal temp is____Agitation is___Doubling is___
A
37C
mild
23 hours
4
Q
What happened in this experiment
A
testing proficiency of CPA’s(glycerol, DMSO)
thaw cells and wash off CPA-centrifugation
stain cells with methylene blue
use hemocytometer to count cells