Lab 1

Question 1

The —-demonstrates a relationship between known concentrations and absorbance. The relationship must be —over range of the standard solutions

Answer 1

standard curve

linear

Question 2

A given cell component is usually colourless for this reason we use

Answer 2

chromogen before spectrophotometry

Question 3

Solute

Answer 3

the soild, solubility increases with temperature

Question 4

What is a saturated solution

Answer 4

a solution that contains maximum amount of solute at a given temperature

Question 5

Concentration is expressed in moles which is —-can also be expressed as

Answer 5

1 mole/L

mass per unit volume mg/L

also percentage this is off 100mL

Question 6

Spectrophotometer is used to measure the—

Answer 6

absorbance

Question 7

Beer-Lambert Law is—-and is calculated as

Answer 7

the absorbance is proportional to the concentration of the absording solution and to the length of the light path

A=kLC
a=absorbance
k=wavelength
L=length of light
C=concentration