Lab

Question 1

Gram stain procedure and what Gram + and Gram - will look like

Answer 1

Apply material to slide, dry

Crystal violet 1 min, wash

Lugol’s iodine 1min, wash

Alcohol decolorizer 10-20 sec, wash

Safarin counterstain 10-45 sec, wash and dry

Gram + : purple, Gram -: pink

Question 2

Blood agar

Answer 2

Common general isolation media, not selective

Differential for hemolytic action

Question 3

Brain Heart Infusion agar

Answer 3

General purpose medium for growth of fastidious microorganisms, especially fungi and yeast

May see pigmentation

Question 4

MacConkey agar

Answer 4

Selective for Gram - (but not all Gram - will grow)

Lactose fermenters are pink

Non-lactose fermenters are colorless

Bile salts may be precipitated

Question 5

Mannitol Salt agar

Answer 5

Fermentation of mannitol causes color change to yellow

Question 6

Typical appearance of microorganisms of chrome agar

Answer 6

E. Coli- pink/red

Klebsiella, enterobacter- metallic blue

Staph aureus - golden, opaque

Staph saprophyticus - pink, opaque

Enterococcus - turquoise

Proteus miriabilis - brown halo

Citrobacter - blue with red halo

Question 7

DNAase Test

Answer 7

Correlates with coagulase in staphylococci

Clearing of dye indicates +

Question 8

Pseudomonas F agar

Pseudomonas P agar

Answer 8

Differentiates pseudomonas aeurginosa from other pseudomonas

Question 9

Primary site of distemper virus replication

Answer 9

Tonsils

Question 10

Main difference between dog and cat herpesvirus

Answer 10

Cat herpesvirus mainly affects the eyes

Question 11

Anterior uveitis and retinal hemorrhage seen with which feline virus

Answer 11

FeLV

Question 12

Which feline virus causes urogenital problems

Answer 12

FeLV

Question 13

Feline virus thar can cause pericardial effusion

Answer 13

FIP

Question 14

Main organ system affected by E. Coli?

Answer 14

Urogenital