Lab 6 Eyes

Question 1

What media are used to culture specimens from eyes?

Answer 1

BA - CO2 or O2
CA - CO2
MAC - optional, O2

All incubated at 35C.

Question 2

Is a direct smear performed on eye specimens?

Answer 2

Yes, a direct smear.

PMNs - 10X
Bacteria & yeast - 100X

Question 3

How do quantitate eye specimen culture plates?

Answer 3

Semi-quantitate

Question 4

What are the major pathogens for conjuntivitis?

Answer 4

N. gonorrhea
Chlamydia trachomatis (serotypes D to K)
H. influenzae
S. pneumoniae
S. aureus
Strep. Group A
Moraxella lacunata
Corynebacterium macginleyi
Syphilis (Treponema pallidum)

Question 5

What are the major pathogens for Keratitis?

Answer 5

S. aureus
S. pneumoniae
P. aeruginosa
GC
Acinetobacter spp.
Herpes simplex virus,
Fungus
Acanthamoeba (parasite)

Question 6

Does the eyes have a normal flora?

Answer 6

Not really, in theory they are sterile.

Transient flora (from skin in small amounts):
S. auerus
Corynebacterium spp.
Cutibacterium acnes,
S. epidermis,
Streptococcus spp.

Question 7

Do conjunctival swabs get AST performed on them?

Answer 7

Not routinely as antibiotics tests for AST are meant for medications that are distributed through blood.

Question 8

What are critical conditions that require critical reporting and AST?

Answer 8

Organisms that cause endophthalmitis and Keratitis.