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AP Biology > Lab 6 > Flashcards

Lab 6 Flashcards

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AP® Biology flashcards Biology 101 flashcards
1
Q

What does a respirometer calculate

A

Rate of oxygen consumption or cellular respiration

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2
Q

What type of seed is used

A

Pea seeds

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3
Q

Seed germination

A

Whether a seed has broken dormancy

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4
Q

What is reviewed and applied for this lab

A

Ideal gas law

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5
Q

What are individual cells responsible for

A

Energy exchanges necessary to sustain ordered structure

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6
Q

How do cells do necessary energy exchanges

A

Breaking down nutrient molecules to generate ATP

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7
Q

What does cell respiration require

A

Nutrient molecules and oxygen

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8
Q

What is cell respiration

A

Breaking down nutrient molecules to generate ATP to fuel cell processes

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9
Q

What are products of cell respiration

A

Carbon dioxide and water

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10
Q

Equation for cell respiration

A

C6H12O6 + 6O2 –> 6CO2 + 6H2O

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11
Q

Ways to measure rate of cell respiration

A

Monitoring changes in temperature

Measure oxygen consumption or carbon dioxide production

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12
Q

Why can temperature be used

A

Respiration is Exergonic and produces heat

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13
Q

What is a respirometer

A

Devices that measure gas volume changes

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14
Q

What is needed to use a respirometer

A

Ideal gas law

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15
Q

Ideal gas law

A

Describes relationship between temperature, pressure, and volume
PV=nrT

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16
Q

What gases are changing in volume during cell respiration

A

Oxygen and carbon dioxide

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17
Q

What is happening to oxygen

A

Consumed by respiring cells

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18
Q

What is happening to carbon dioxide

A

Diffusing out

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19
Q

How can the respirometer deal with two gases

A

Potassium hydroxide which absorbs carbon dioxide

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20
Q

KOH formula

A

CO2 + 2KOH –> K2CO3 + H2O

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21
Q

What is potassium carbonate

A

A solid precipitate

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22
Q

What happens to carbon dioxide when potassium hydroxide is added

A

Turns into a solid, so respirometer will only measure consumption of of oxygen

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23
Q

What respirometers will be used and how

A

2 sets of 3, submerged in water
Each set at a different temperature
One with germinated seeds, one with non-germinating seeds and plastic beads, and one with beads
Also contain cotton saturated with KOH

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24
Q

Why the beads

A

To ensure each respirometer is uniform in volume

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25
Q

Why cotton

A

Carbon dioxide will be absorbed

26
Q

What will happen in pan of water

A

Water will flow from high pressure to low pressure
Oxygen used up by seeds, gas pressure inside respirometer will decrease, water will flow into the pipette down pressure gradient

27
Q

What do pictures look like

A 
Pipet tip
Seeds on top
Beads
Dry cotton
Absorbent cotton with KOH
Weights underneath
28
Q

How many of each type of seed

A

50

29
Q

How will the beads be

A

In a container

30
Q

Ho many stoppers

A

6

31
Q

What will be attached to stoppers

A

1 mL graduated pipettes

32
Q

How many paper towels

A

6

33
Q

How many water bath containers

A

2

34
Q

What type of graduated cylinder

A

100 mL

35
Q

How many thermometers

A

2

36
Q

What other materials

A 
Absorbent cotton
No absorbent cotton
Sharpie
Ice
Masking tape
Cell phone timer
Bucket of water
Scissors
Plastic trash bucket
37
Q

What will be in the dropper bottle

A

15% KOH

38
Q

What about respirometer tops

A

Be careful
They’re rubber with pipette sticking out
Will lose seal

39
Q

Step 1

A

Label paper towels 1a, 2a, 3a, 1b, 2b, 3b

40
Q

What is step 2

A

Label respirometer a with sharpie, same as paper towels

41
Q

Step three

A

Fill grad cylinder with 50 mL of water
Put 25 germinating seeds in the cylinder
Record volume in Data Table 1a
Subtract 50 mL to determine total volume of seeds and record same place
Pour out water into trash bucket and put seeds on paper towel 1a

42
Q

What is step 4-5

A

Same as step 3, but after you do 25 dry seeds, count in plastic beads until volume matches
Record same place
Put mixture on 2a

43
Q

Step 6

A

Same as last ones, but add beads till get same volume
Data table 1a
Beads on paper towel 3a

44
Q

What is step 7

A

Do it for the b respirometer said and record in 1b

45
Q

What are the lines at in the pipette

A

Hundredths

46
Q

Step after weighing

A

Put absorbent cotton to bottom of weighted vials, push down with wooden stick or stirring rod
Saturate with 4 drops of 15% KOH

47
Q

About KOH

A

Caustic

48
Q

After absorbent cotton

A

No absorbent cotton, push to bottom

49
Q

Why non absorbent

A

Peas don’t touch poisonous KOH

50
Q

After cotton

A

Add peas and beads
Put in stoppers
Should be tight and not wiggle much

51
Q

Where does part 2 start

A

After filling vials

52
Q

Part 2 step 1

A

Will baths with tap water 1 inch from top
Room temp, and cold temp by adding ice til 10 degrees Celsius and 25 Celsius
Continue adding ice, ask Mallett

53
Q

Second step part 2

A

Masking tape across narrow width of bath, 2/3 way from 1 end

Wither notebook paper in bottom of tub to read pipettes easier

54
Q

Step three part 2

A

Place thermometers on floors of baths, take temps record 1C

55
Q

Step 4 part 2

A

Put a respirometers in room temp water bath so pipette rest on masking tape
Same with b, but put them in cold

56
Q

What to do after placing respirometeso

A

Time for 7 minutes - equilibration period to be same temp as water
Don’t hit bath or table at any point

57
Q

Start time then

A

Mrs. Mallet show you how to put in food coloring

58
Q

Where will lab need to be fast

A

After food coloring

59
Q

Fast part

A

At end of 7 minutes, put tubes entirely in baths
Cut tape ends so respirometers go in gently
Some water gets in pipettes, but influx should stop quickly, if it continues check for leaks and call for Mallett

60
Q

Second step fast

A

Make sure you can read scale
Air bubble should extend from main chamber up tube of pipette
Record water level onto the data table at time interval 0
Record position of water in each pipette 5, 10, 15, 20 min data table 2

61
Q

Clean up

A

Due respirometers onto paper towel and rinse beads put them back in container from Mallett so they dry
Throw out peas
Scissors to pull out cotton and throw away
Slide water tubs toward sink and dump water - careful
Sham wow dry water from pans
Rinse pipettes with distilled water
Once water is clear,s hake pipette over sham wow to remove as much water as possible and return pipette where instructed, don’t break it or its seal

62
Q

Two questions

A

How temp and seed germination affect rate of respiration

AP Biology (4 decks)

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