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Microbiology Lab > lab 5 > Flashcards

lab 5 Flashcards

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Biology 101 flashcards
1
Q

Eukaryotes

eukaryotic cell type; Domain Eukarya

A

Fungi

Protozoans

Helminths

Algae

Some members of these groups are human pathogens.

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2
Q

Types of fungi

A

Yeasts Molds Mushrooms

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3
Q

Sabouraud dextrose agar (SDA) plate contents

A

Selective agar used for growing most molds and yeasts

high glucose (dextrose) conc. – preferred by fungi.

acidic environment (pH 5.6) - inhibits most bacteria.

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4
Q

Yeast examples and characteristics

A

Examples: Candida albicans, Saccharomyces cervisiae

Yeast shapes vary; ovoid shaped are common

Note size, buds (aka blastospore) and bud scars

About 10x bigger than bacteria (e.g., cocci)

Buds form on parent cell

Parent cell is scarred at release site

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5
Q

Typical Mold Colony

Vegetative Mycelium characteristic

A

Whitish edge growth reveals underlying vegetative mycelium

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6
Q

Typical Mold Colony

Vegetative Mycelium reproductive structures

A

Darker area shows mold spores produced from underlying reproductive structures

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7
Q

Typical Mold Colony

Vegetative Mycelium comprised of?

A

Comprised of interwoven hyphae - long branching filaments

appear fuzzy

Typically appears whitish

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8
Q

Typical Mold Colony
Vegetative Mycelium
Fruiting Bodies (Reproductive structures)

A

Form at various locations along vegetative hyphae

Morphology of structures vary among species

Spores typically give the color (e.g., green, black)

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9
Q

Septated Hyphae

A

Filaments contain porous septa.

Pore allows cell materials to move.

Cells may be multinucleated

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10
Q

Non-Septated Hyphae

A

Lacks septa

multinucleated

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11
Q

Conidiospores are often called conidia; occur?

A

occur in chains

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12
Q

Asexual Mold Spores, 3 types

A

Aspergillus- condiospores

Penicillium - condiospores

Rhizopus - sporangiospores

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13
Q

Potential health issues related to common household molds

A

Molds spores are very light and disseminate rapidly in the air.

Some people are sensitive to molds.

Symptoms can include nasal stuffiness, eye irritation, wheezing, or skin irritation

More severe reactions in some include fever and shortness of breath, and potential mold infections in their lungs.

Mold spores are much easier to kill than bacterial endospores

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14
Q

Protozoans - eukaryotes

Protozoan diseases include

A

Trichomoniasis, Amoebiasis, PAM, Giardiasis, Toxoplasmosis

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15
Q

Protozoans - eukaryotes

Protozoans include non-pathogens and pathogens

A

Non-pathogen  Euglena – using hanging drop technique

Pathogen  Trichmonas vaginalis – observe prepared slide

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16
Q

Methods to Determine Motility

A

Flagella stain

SIM

Wet mount

Hanging drop slide (best method)

17
Q

Typical Mold Asexual Reproductive Life cycle

A
  1. Asexual spores
  2. Spore germination
  3. Initial growth of vegetative hyphae
  4. Vegetative Mycelium development
  5. Reproductive Structures (aka Fruiting Bodies  produce spores)
18
Q 
Compare and contrast Candida albicans vs Trichomonas vaginalis
Diseases caused?
Similarities?
Transmission?
Signs and symptoms?
Treatment?
Prevention?
A

Candida albicans:
Disease: Volvovaginitis (gram stain). Treatment: Nystatin, clotrimazole
Trichomonas vaginalis:
Disease: Tricomaniasis (wet prep). Treatment: metronidazole
Similarities: BOTH cause vainitis, can be spread sexualty, are eukarotic
Transmission: BOTH sexually transmitted
Signs/symptoms: BOTH itching, burning, and white discharge
Prevention: NO SEX!!

19
Q

What type of specimen would you collect to diagnose?

tinea barbae?

A

skin scrapping from the beard (chin) area

20
Q

What type of specimen would you collect to diagnose?

thrush?

A

oral cavity, white patches on mucous membranes

21
Q

What type of specimen would you collect to diagnose?

cryptococcosis?

A

lung tissue

22
Q

What type of specimen would you collect to diagnose?

toxoplamosis?

A

plasma (antibody test)

23
Q

What type of specimen would you collect to diagnose?

blastomycosis?

A

chest xray, skin biopsy, sputum culture

24
Q

Four classes of protozoa?

A

amoeba,, flagellates, ciliates, sporoza

25
Q

Describe Sabouraud Dextrose Agar (SDA) plate

A

media choice for growing mold and yeast
contain increased amounts of glucose and lower acidity around pH 5-6
lower acidity inhibits many bacteria
molds are not streak plated on SDA (use inoculating needle)
yeast uses streak plate

26
Q

What are molds composed of?

A

filaments called hyphae which interweave to form the vegetative (non reproductive) mycelium

27
Q

What develops within the vegetative mycelium?

A

foot cells develop from which asexual reproductive structures will develop. These reproductive structures are also referred to as fruiting bodiesYeas

28
Q

Yeast characteristics? Most common?

A 
non motile
unicellular fungi that reproduce asexually by forming buds (blastospores)
eukaryotes
circular, ovoid and rod shaped
5x bigger than bacteria
most common: Candida albicans
29
Q

Protoxoan characteristics?

A

eukaryotic

classified according to the structures they use for motility

30
Q

Which of the following media has an acidic pH and increased glucose?

a. Sabouraud dextrose agar (SDA)
b. Chocolate agar (CA)
c. MacConkey agar (MAC)
d. Nutrient agar (NA)

A

Sabouraud dextrose agar (SDA)

Feedback:

Sabouraud dextrose agar is well suited for cultivating molds and yeats. It provides extra glucose (dextrose), an acidic pH of 4-5, and the humidity required for optimal growth of most fungi.

NOTE: Many bacteria prefer a neutral pH in a growth medium.

31
Q

In molds, asexual reproduction uses a fruiting body to produce ______.
Select Answer:

a. ova
b. spores
c. rods
d. Blastospores

A

spores

32
Q

Which branch of science deals specifically with the study of yeasts, molds, and mushrooms?
Selected Answer:

a. Epidemiology
b. Parasitology
c. Mycology
d. Virology

A

Mycology

33
Q

Of the following microbes which one is considered a eukaryotic cell?

Staphylococcus aureus
Escherichia coli
Candida albicans
Klebsiella pneumoniae
Pseudomonas species
Proteus vulgaris
A

Candida albicans

34
Q

Mycelium that extends up off a surface such as an agar medium is referred to as-

a. vegetative mycelium
b. aerial mycelium
c. aggregate mycelium
d. segmented mycelium

A

aerial mycelium

IS THIS CORRECT????? FROM PRE-LAB 5 QUIZ

35
Q

Bacterial growth in a nutrient broth that appears particulate is referred to as what type of growth?

a. Turbid
b. Favorable
c. Standard
d. Flocculent

A

Flocculent

Microbiology Lab (12 decks)

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