lab 12 Flashcards
What are safe cooking temperatures for the following: Beef roast/steaks Fish Pork Ground beef Egg dishes Chicken (whole or ground) Turkey (whole or ground)
Beef roast/steaks 145 Fish 145 Pork 145 Ground beef 160 Egg dishes 160 Chicken (whole or ground) 165 Turkey (whole or ground) 165
What color is produced for the following:
E. coli - MAC and HE
Salmonella sp. - MAC and HE
E. coli MAC- beige/pink HE- orange Salmonella sp. MAC- green HE- beige
Define Enterics and other info
Normal flora of the intestines of human and/or animals
- > 90% are obligate anaerobic bacteria
- includes bacteria, fungi, protozoans, etc
- normal flora are predominately non-pathogens
- Enterobactraceae - a bacterial family with many GNB enterics (Salmonelle, Shigella, E. coli, Proteus
Name 2 clinical manifestations of GI disease
Diarrhea- mainly watery stools, typically no blood
Dysentery- more severe; often higher volume water stools, mucus, pus, blood
-may include fever, cramps, pain and vomiting
True or False
Gastrointestinal disease can consist of infections and/or intoxications
True
Name 4 bacterial pathogens (Enterobacteraceae family) of the GI? Gram reactions? Disease type?
Salmonella (animal flora), GNB, infection
Shigella (animal flora), GNB, infection
Escherichia coli (E. coli 0157:H7 is animal flora), GNB, infection
Proteus sp., GNB, infection
MacConkey Agar (MAC) plate contents
Selective and Differential for growing many GN’s
Low bile & CV dye- inhibits GP’s
Contains lactose
-lactose fermenters = pink colonies
-non fermenters = colorless or beige colonies
What bacteria ferments lactose on a MAC plate?
Escherichia coli
Serratia
Klebsiella
What bacteria is a non fermenter on a MAC plate?
Proteus
Salmonella
Shigella
Hektoen Enteric (HE) plate contents
Selective and Differential (more selective than MAC)
-used to better isolate more pathogenic enterics
Higher bile and dyes (bromothymol blue, acid fushin)
-inhibits GP and many GN
Contains lactose, sucrose, salicin
-fermenters appear yellow, orange, salmon or pink
-non fermenters appear green/blue
Contains sulfates + iron (like SIM agar)
-sulfide producers form colonies with black centers (H2S)
Name 3 bacteria that produce black centers (H2S) on an HE plate
Salmonella sp.
Escherichia coli
Proteus vulgaris
What is the expected Gram reaction of a member of the Enterobacteriaceae?
Gram negative
What is the oxygen requirements of the family Enterobateriaceae?
Facultative anaerobic
What is the oxygen requirements for most of the intestinal enteric bacteria?
Most are obligate anaerobic, some aerobic
List 5 genera found in the Enterobacteriaceae
Salmonella Escherichia coli Salmonella typhi Shigella Proteus sp.
Escherichia coli 0157:H7 on HE plate Gram reaction statement? HE agar fermentation? ID Disease? Clinical manifestations? Treatment?
GNB
Fermentor with yellow/orange colonies
10-100 cells
Gastroenteritis, dysentary, hemolytic eremic syndrome, UTI, menigitis, septicemia
Cramping, diarrhea, vomiting, fever
HUS- decreased urination, lethary, decreased kidney function
Supportive only, fluids, electrolytes
Shigella sonnei or flexneri on HE plate Gram reaction statement? HE agar fermentation? ID Disease? Clinical manifestations? Treatment?
GNB
Non fermenters, clear colonies. No sulfide produced
10-200 cells
Shigellosis or bacillary dysentery
Bloody diarrhea, fever, cramping
Ampicillin, ceftriazone, trimethoprim-sulfamethoxazole
Salmonella enteritis on HE plate Gram reaction statement? HE agar fermentation? ID Disease? Clinical manifestations? Treatment?
GNB
Non fermenter, sulfide producers blue w/ black center
12-20 cells
Salmonellosis, typhoid fever
Fever, ab pain/cramping, diarrhea
Ampicillin, trimethophrim-sulfamethoxazole, ciprofloxin and supportive care, fluids/electrolytes
Salmonella typhi on HE plate Gram reaction statement? HE agar fermentation? ID Disease? Clinical manifestations? Treatment?
GNB
Non fermenters, sulfide producer blue w/ black center
1000-10000 cells
Typhoid fever
Weakness, nausea, vomiting, ab pain, high fever, possible ab rash
Ampicillin, trimethophrim-sulfamethoxazole, ciprofloxin and supportive care, fluids/electrolytes
