Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Bacteriology Labs > Lab 4 > Flashcards

Lab 4 Flashcards

1
Q

Corynebacterium habitat

A

Mucous membranes, skin, soil, milk (animal pathogens - saprophytes). There are some plant pathogenic

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Corynebacterium Morphology

A

Polymorphism, very different shapes appear in one smear – Rods (typically curved), coccus. Chinese letters.
Metachromatic granules, stain strongly and contain stored materials.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Corynebacterium culture

A

Blood agar, haemolysis. Weak growth on nutrient agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Corynebacterium, resistance

A

Average, short time in the environment.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Corynebacterium Pathogenicity

A

Local suppuration, arthritis, metritis, mastitis, abscesses.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Imp spp coryne

A

C. diphtheriae: Hu pathogene, diftery (inflammation of throat)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Nocardia habitat

A

Soil

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Nocardia Morphology

A

Long, branched filaments, wrinckled colonies.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Culture Nocardia

A

Obligate aerobic, Carotenoid pigment, Filaments

- Nutrient agar (not fastidious), 3-5 days to form a colony.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Nocardia Pathogenicity

A
  • Facultative pathogens, local lesions, - N. asteroides:

- Cattle chronic suppuration - Mastitis in cattle

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Actinomyces habitat

A

Alimentary tract, mucous membranes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Morphology Actinomyces

A

Branching filaments, broken, sulphur granules in the pus but not on medium

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Actinomyces Culture

A

Anaerobic or facultative anaerobic, blood agar.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Actinomyces Species and diseases

A

A. bovis: cattle actinomycosis - lumpy jaw, pig udder - actinomycosis

A. viscosus: caryes in human, dog – actinomycosis

A. israeli: obligate anaerobic human actinomycosis

A. hordeovulneris: dog actinomycosis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Dermatophilus

congolensis Habitat

A

Soil, skin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

morph Dermatophilus

congolensis

A

Coccoid with branching filaments which can break into coccoids.

17
Q

Dermatophilus

congolensis Culture

A

Obligate aerobic and capnophilic (need CO2), fastidious, blood agar. Form wrinckled yellow colonies. Hemolysis.
Life cycle: Flagellated coccus (zoospora) - filaments - branching - growth - cracking - zoospora

18
Q

Dermatophilus

congolensis biochem

A

Proteolytic

19
Q

Dermatophilus

congolensis pathogenicity

A

Exsudative dermatitis in mainly: Sheep, cattle

20
Q

Renibacterium salmoninarum habitat

A

Water, fish

21
Q

Renibacterium salmoninarum morph

A

1-1,5 μm rods, short chains

22
Q

Renibacterium salmoninarum culture

A

Aerobic, 15-18 oC (since it affects fish) Fastidious (cistein, serum)

23
Q

Renibacterium salmoninarum pathogenicity

A

Obligate pathogenic, intra cellular

Salmon, trout: abscesses in kidneys and other organs

24
Q

Rhodococcus

R. (C.) equi habitat

A

Soil, plant, gut of horse

25
Q

Rhodococcus

R. (C.) equi morph

A

Coccus-rod cycle. At the beginning of replication: rods and later
Capsule of polysaccharide.

26
Q

Rhodococcus

R. (C.) equi culture

A

Simple Pigment (pink) Capsule

27
Q

Rhodococcus

R. (C.) equi pathogenicity

A 
Foal pneumonia
Human pneumonia (immunosuppression, AIDS)
28
Q

Mycobacterium habitat

A

Infected host, shed with faeces, milk etc. to the environment.

29
Q

Mycobacterium morph

A
  • Rods, coccoids
  • Cell wall has lipids and waxes with mycolic acids. - No flagella, spores or capsule
  • Species differences
30
Q

Mycobacterium stain

A
  • Ziehl-Neelsen positive (Acid fast)
  • Theoretically: Gram positive but colorless since the dye can’t go through the cell wall. - Fluorescence dyes (auramin staining
31
Q

Mycobacterium Culture

A

Obligate aerobic

  • Broth: Henley, Sauton (cord formation is characteristic for virulent bacteria). Usage of tube instead of solid medium due to the long propagation time. Media goes dry after a couple of days.
  • Solid:
  • Egg (Kertai, Dorset, Petragnani and the most common Löwenstein-Jensen.) Egg enriched media for the pathogenic spp.
  • Serum agar, glycerine potato.
  • Selective: Petragnani, acid treatment (5% oxalic acid 10 min, neutralize with a base and then centrifuge and use the sediment for inoculation), animal trial.
  • Incubation time, growth characteristics (species differences)
32
Q

Mycobacterium antigens

A

Glycolipids, lipoproteins

  • Shared antigens, immuno electrophoresis used to examine.
  • Tuberculin (protein characteristic for M.) – PPD (purified protein derivate)
33
Q

Mycobacterium resistance

A

Good against chemical disinfectants and environmental influences but not heat. Has the best resistance among the Gram + non-sporeforming bacteria.

34
Q

Mycobacterium Pathogenicity

A
  • Obligate pathogens
  • Facultative pathogens
  • Saprophytes (atypical)
    Most common diseases are tuberculosis, paratuberculosis and Fe leprosy.

M. tuberculosis: human, swine, dog, cat, parrot, cattle
M. africanum: human
M. bovis: cattle, ruminants, swine, dog, cat, human, BCG (Bacillus Calmette-Guerin, 1
M. bovis strain as vaccine for Hu, not animals. Inoculation for 13 years on potato
media and strain lost its virulence.)
M. leprae: human
M. lepraemurium: mouse, cat (not culturable)

35
Q

Mycobacterium groups

A

Groups – based on pigment production and growth

36
Q

Mycobacterium: Photochromogens

A

pigment prod. in light: M. kansasii, M. marinum

37
Q

Mycobacterium Scotochromogens

A

pigment prod. in light and dark: M. scrofulaceum, M. gordonae

38
Q

Mycobacterium Non-chromogens

A

no pigment prod.: M. intracellulare, M. terrae

39
Q

Rapid growers mycobacterium

A

form colonies after several days: M. phlei, M. fortuitum

Bacteriology Labs (4 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions