lab 3 Flashcards
components of fixative
ethanol, acetic acid, water
purpose of fixative
- fix proteins in place
- allow for better staining
purpose of this lab
to stain fish gels and analyze them
what is the staining solution called?
Coomassie blue
components of Coomassie blue
Coomassie brilliant blue G250, phosphoric acid, methanol, water
true or false: you need a labeled picture of your gel in your notebook
true
what kind of graph are we creating
a standard curve
what is our standard for protein size
Precision Plus Protein Kaleidoscope
we should analyze and measure proteins between which sizes?
50 kD and 10 kD
what is our accuracy when measuring
0.5 mm
how do we measure bands
measure from bottom of the well to the middle of the band
what is on the x-axis
molecular weight (kD) (log scale)
what is on the y-axis
distance traveled (mm), regular scale
true or false: our line of best fit will be linear
false
how many different tables should we be making
5 dif tables for each fish, each with at least 8 proteins
what will we construct at the end of lab?
cladogram
