8.18-8.20 Flashcards

1
Q

TGN is responsible for sorting proteins into vesicles destined for where?

A

apical vs. basal membranes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

basolateral vesicle signal

A

tyrosine and dileucine-based signals in cytoplasmic domain

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

apical vesicle signal

A

no specific cytoplasmic domain signals
- have N/O-linked sugars in lumen domain, or GPI anchors

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

recycling endosome

A

subset of early endosome, also capable of sorting molecules

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

regulated secretion from TGN uses _____________

A

secretory granules

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

signal for secretion

A

secretagogue

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

synaptotagmin

A

Ca2+-binding protein that is key player in fusion

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

what does synaptotagmin do

A

regulates vesicle secretion by binding to SNARE pairs and holding them in inactive configuration and prevents fusion

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

what happens to synaptotagmin when nerve impulse triggers release of Ca2+

A

Ca2+ changes conformation of synaptotagmin, causes it to let go of SNARE complexes, allows membrane fusion

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

where can ABC transporters be found

A

ER, Golgi, PM

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

what does ABC stand for

A

ATP-binding cassette

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

where do ABC transporters bind protein

A

c-face

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

what energy do ABC transporters use

A

ATP energy

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

ex. of proteins that move by ABC transport

A

interleukins, FGF

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

types of ABC transporters and uses

A
  • GRASP
  • CFTR
  • MHC class I antigen presentation
  • MDR protein
  • chloroquine-resistance
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

GRASP

A

Golgi-membrane associated protein - grabs protein from c-face, transports into Golgi

17
Q

role of ABC transporters in MHC class I antigen presentation

A
  • peptide fragments in cytosol from protease activity
  • ABC gets into ER for association with MHC class I
  • T-cytotoxic lymphocytes can be activated
18
Q

CFTR

A

Cl- channel

19
Q

where is chloroquine-resistance found

A

Plasmodium falciparum

20
Q

chloroquine-resistance

A

chief cause of malaria, resistance due to ABC transporter exporting drug

21
Q

what do tetanus and botulinum toxin both target

22
Q

tetanus and botulinum toxin are both:

A

zinc proteases

23
Q

tetanus toxin

A

causes spastic paralysis:
- inhibits release of inhibitory neurotransmitter GABA by cleaving SNAREs in inhibitory interneurons and preventing vesicle docking

24
Q

botulinum toxin

A

causes flaccid paralysis:
- cleaves SNAREs in neurons and prevents release of acetylcholine from neuromuscular junctions

25
Q

one of SNARE types targeted by tetanus and botulinum toxin

A

synaptobrevin