LAB 240 Exam 1 Mycology Flashcards
List the various tineas with the part of the body affected. (for example, tinea capitis- scalp).
Ungium=Nail Corpus=Body Petus=Foot Barbe=Beard Cruris=groin
What does the word “tinea” mean?
Latin word for Ring Worm
Name the short lateral structure that will develop when certain yeast are incubated at 37 in a rich medium
germ tube
Name one yeast that is germ tube positive
Canada albicans
At what temperature should chlamydospore agar be incubated?
25 or room temp
Where should the technologist look for spores on a chlamydospore agar plate?
on the edge of the cover slip
What color and shape are they
Blue and round
What is the principle of the India ink preparation?
take a drop of india ink and a drop of organism and under high power u will see a clear halo around the organism, its a budding yeast and you will see a clear halo, because the india ink bounces off the capsule and creates a halo around the organism
What organism is India Ink especially used for
Cryptococus Neoformans
What type of specimen is most frequently used
Spinal Fluid
What reagent should be used for the examination of the reproductive structures of molds that are already growing in a culture?
Lactophenol cotton blue
LPCB
What technique can be used to “save” wet preps for future use?
clear finger nail polish around the cover slip or parafilm
Name several media used for the primary isolation of fungi.
Sabouraud's (Sabs) Sabs C SDA (Sabouraud's Dextros Agar) BHI (Brain Heart Infusio) Mycoseal Mycobiotic BHT
Why would you incubate a culture for fungi at 25and 37?
grows as mold at 25
and yeast at 37
What term is used for fungi that grow differently at these temperatures
dymorphic
