Lab 2: Microscope Skills

Question 1

compound microscope

Answer 1

A type of microscope in which light passes through two different magnifying lenses before it gets to your eye

Question 2

magnification

Answer 2

How many times larger (“compounded”/ multiplied) the object appears to be

Question 3

resolution

Answer 3

The ability to distinguish two objects from each other under the microscope

Question 4

resolving power

Answer 4

A measure of the ability to distinguish two objects as separate

Question 5

oil immersion lens

Answer 5

A microscope technique used to enhance resolution by putting oil between the microscope’s lens and specimen so that more light is on the specimen.

Oil makes the light from the microscope bend less, so more light is on the specimen

Question 6

ocular lens

Answer 6

Eyepiece of the microscope with a magnification value of 10x

Question 7

binocular head

Answer 7

Name of the two ocular lens plus the tubes together

Question 8

arm

Answer 8

Connects the binocular head to the base of the microscope

Question 9

revolving nosepiece

Answer 9

Rotates to move the objective lens into place

Question 10

objective lens

Answer 10

The lens closest to the slide.

Has magnification values around the edge.

Question 11

power switch

Answer 11

Turns light source on/off

Question 12

light intensity knob

Answer 12

Controls the intensity of the light passing through the slide

Question 13

longitudinal adjustment knob

Answer 13

Controls slide movement from front to back

Question 14

transverse adjustment knob

Answer 14

Controls slide movement from side to side

Question 15

coarse adjustment knob

Answer 15

Used BEFORE fine adjustment to initially focus the specimen. Turning the coarse knob causes the stage to move up and down very quickly

Question 16

fine adjustment knob

Answer 16

“fine focuses” the image

Question 17

lamp

Answer 17

provides a light source

Question 18

base

Answer 18

Bottom of the microscope

Question 19

iris diaphragm lever

Answer 19

controls the amount of light transmitted by opening/closing

Question 20

condenser lens

Answer 20

focuses and concentrates light on the specimen

Question 21

stage

Answer 21

the flat structure of the microscope which the microscope slide is placed on

Question 22

stage clips

Answer 22

mechanism that holds the slide in place

Question 23

pointer

Answer 23

right ocular tube may have a pointer- this allows you to move a slide and line up a specimen or structure with the pointer. Visible when looking through ocular lens

Question 24

condenser height adjustment knob

Answer 24

moves condenser (and iris diaphragm) up and down. The condenser lens may be moved at any time to improve the contrast and illumination of the slide

Question 25

total magnification

Answer 25

Objective lens value multiplied by the ocular lens value

Question 26

eyepiece

Answer 26

Where you view the specimen, consists of the ocular lens and tube

Question 27

binocular tubes

Answer 27

Tube that connects the ocular lens to the binocular head

Question 28

parfocal

Answer 28

When the lens stays in focus when magnification/focal length is changed.

Question 29

As magnification increase, what happens to the microscope’s field of view for the slide

Answer 29

The field of view decreases as magnification increases

Question 30

field of view

Answer 30

diameter that is seen of the specimen based on total magnification

Question 31

depth of field

Answer 31

The distance from the nearest object plane in focus to the farthest object plane that is simultaneously in focus

Question 32

As magnification increase, what does that do to depth of field?

Answer 32

Like FOV, depth of field also declines as magnification increases.

Question 33

wet mount

Answer 33

a glass slide holding a specimen suspended in a drop of liquid (as water) for microscopic examination

Question 34

How many micrometers are in 1 millimeter (mm)?

Answer 34

1 millimeter = 1,000 micrometers