Lab 2

Question 1

What is a pure culture

Answer 1

Culture containing a single species of an organism. It is free from contamination by any other organisms.

Question 2

What is a sterile medium

Answer 2

A nutrient rich solution free from living microbes. It provides a safe environment for microbes to grow without contamination

Question 3

What is a inoculum

Answer 3

Small sample of micro organisms introduced into a sterile medium to initiate growth

Question 4

What is the aseptic technique

Answer 4

Involved procedures used to prevent contamination by unwanted microbes during laboratory work

Question 5

What is pellicle broth growth

Answer 5

A layer of growth at the surface of the broth due to microbes getting oxygen

Question 6

What is sediment growth broth

Answer 6

Growth that settled at the bottom of broth, representing organisms that don’t need or want oxygen conditions

Question 7

What is turbidity broth growth

Answer 7

Cloudiness throughout broth, representing organisms that can take both oxygen and no oxygen

Question 8

What is a obligate aerobe

Answer 8

Microbes that thrive and need oxygen to survive

Question 9

What is a obligate anaerobe

Answer 9

Microbes that grow only with no oxygen

Question 10

What is a facultative anaerobe

Answer 10

Microbes that grow with or without oxygen but prefer oxygen

Question 11

What is a aero tolerant anaerobe

Answer 11

Organisms that don’t require oxygen but can tolerate it without being harmed

Question 12

What is a microareophile

Answer 12

Organisms that need only low oxygen and with too much oxygen they can be harmed

Question 13

What is a psychrophile

Answer 13

Organisms that thrive at very low temperatures, typically between -20°C and 10°C.

Question 14

What is a mesophile

Answer 14

Organisms that grow best at moderate temperatures, usually between 20°C and 45°C.

Question 15

What is a thermophile

Answer 15

Organisms that prefer high temperatures, typically between 45°C and
80°C.

Question 16

What is a hyperthermophile

Answer 16

Organisms that thrive at extremely high temperatures, usually above 80°C, such as those found in hot springs or deep-sea hydrothermal vents.

Question 17

Define Chromogenic:

Answer 17

Refers to microorganisms that produce colored compounds or pigments during growth.

Question 18

Define water soluble pigments

Answer 18

A pigment that can dissolve in water and diffuses throughout the medium, coloring the surrounding liquid.

Question 19

Define insoluble

Answer 19

A pigment that does not dissolve in water and remains localized to the colony or the microorganism itself without diffusing into the surrounding medium.