Lab 2 Flashcards
Pure Culture
A single species or strain of bacteria growing in culture , cloned identical cell colonies
Most common procedures to for isolation
Isolation or quadrant steak method
Failure to flame between quadrants
Would not create a isolation streak because the flame is one of the ways to reduce bacteria throughout process
Streaks not parallel or over lapping
you will pull too many cells into the next area and will not reduce the number enough to get isolated colonies
Transfer from one place of living to a new place
Sub culture
is a common culture medium inoculated by a straight streak of organism from bottom to top with either a loop or needle,
Slant medium
Characteristics observed on slant culture
Amount
Color
Shape
Opacity ( transparency, translucent)
Odor
Uniform dispersion of the bacteria within the liquid will lead to, or cloudiness.
Middle of broth
Turbidity
Some organisms will settle out, or fall to the bottom of the tube.
Sediment
Another character to look for in broths is the presence of a pellicle, or skim, on the surface of the broth. (If the broth is agi-tated, this will often fall.)
Top
Pellicle
Isolated colonies
Size
Color
Margin
Shape
Elevation
A temperature at which the enzyme functions at its highest rate
Optimal temperature
The structure enzymes is altered it may no longer attach to appropicate substrate
Denature
Bacteria species that will grow in a range from 5 up to 20
Organisms live in cold water soils and fridge
Psychrophiles
The only human pathogen that grownphsychrophilically listeria
monocytogenes
