Lab 2 Flashcards
Pure Culture
A single species or strain of bacteria growing in culture , cloned identical cell colonies
Most common procedures to for isolation
Isolation or quadrant steak method
Failure to flame between quadrants
Would not create a isolation streak because the flame is one of the ways to reduce bacteria throughout process
Streaks not parallel or over lapping
you will pull too many cells into the next area and will not reduce the number enough to get isolated colonies
Transfer from one place of living to a new place
Sub culture
is a common culture medium inoculated by a straight streak of organism from bottom to top with either a loop or needle,
Slant medium
Characteristics observed on slant culture
Amount
Color
Shape
Opacity ( transparency, translucent)
Odor
Uniform dispersion of the bacteria within the liquid will lead to, or cloudiness.
Middle of broth
Turbidity
Some organisms will settle out, or fall to the bottom of the tube.
Sediment
Another character to look for in broths is the presence of a pellicle, or skim, on the surface of the broth. (If the broth is agi-tated, this will often fall.)
Top
Pellicle
Isolated colonies
Size
Color
Margin
Shape
Elevation
A temperature at which the enzyme functions at its highest rate
Optimal temperature
The structure enzymes is altered it may no longer attach to appropicate substrate
Denature
Bacteria species that will grow in a range from 5 up to 20
Organisms live in cold water soils and fridge
Psychrophiles
The only human pathogen that grownphsychrophilically listeria
monocytogenes
Bacterial species which grow within the range from just below 20- up to 45
Humans and other organisms live
Mesophiles
Growing form 20-35 these are plants pathogens
Decomp of plants
Room temp mesophiles
Bacterial species which have thermostable enzymes flourish in hot environment
Growing at 45 and higher
Thermophiles
Found on warm blooded animals as mutualistic normal flora or pathogens 35-45
Body temp mesophiles
Rapidly attach two molecules and change them into harmless water and 02 molecules
Catalase
Flexiable group of bacteria based on oxygen
Facilitative
Small air loving
Microaerophiles
Blue strip
Gas pak growth of microaeropjoles in jar
Anaerobe system
Anaerobic and facultative
Candle jar
Anaerobic and facultative
Candle jar
Chemicals used on living surfaces such as skin to kill or inhibit the growth of bacteria
Antiseptics
Too harsh for living tissues and instead used to kill or inhibit microbes growth on non living surfaces
Disinfectant
Chemicals used in the treatment of disease rather than just to inhibit or kill
Antibiotic
A sense of growth around the disk by clear area called
Zone of inhibition
