Lab 14 Flashcards
1
Q
What is gel electrophoresis?
A
movement of a charged particle in an electric field
2
Q
What was the concentration of the gel used in the experiment?
A
1.5%
3
Q
Is DNA positive or negative?
A
negative
4
Q
What happens to DNA in electrophoresis?
A
It moves from negative to positive
5
Q
Do smaller DNA fragments migrate slower or father then larger ones?
A
Faster
6
Q
The bands formed in electrophoresis are based on what?
A
size
7
Q
1.5% Agarose gel is capable of separating how many base pairs of DNA?
A
200-3,000
8
Q
How many base pairs can a 0.3% gel separate?
A
5,000-60,000
9
Q
How many micro-mL are in a mL?
A
1000
10
Q
What was the range (in micrometers) that the pipets could transfer?
A
2.0 to 20.0mL
11
Q
What is the equation for % solution?
A
X grams / 100 mL
12
Q
What were the volts used in this experiment?
A
100 and 150
