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Biology Laboratory > Lab 10: Photosynthesis > Flashcards

Lab 10: Photosynthesis Flashcards

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AP® Biology flashcards Biology 101 flashcards
1
Q

The ability to convert solar energy into chemical energy is a trait of what?

A

autotrophs

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2
Q

What is a by-product of photosynthesis?

A

oxygen

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3
Q

What is the molecular formula of photosythesis?

A

6 CO2 + 12 H2O uses light energy to convert to C6H12O6 + 6 H2O and 6 + O2

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4
Q

What are the reactants of photosynthesis?

A

Water and Carbon Dioxide

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5
Q

What are the products of photosynthesis?

A

Glucose
Water
Oxygen Gas

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6
Q

Where does photosynthesis occur?

A

The chloroplasts

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7
Q

Where are pigments located?

A

in the thylakoid phospholipid bilayer

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8
Q

What are the four types of pigments used in this lab?

A

Chlorophyll A
Chlorophyll B
Carotene
Xanthophylls

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9
Q

Heavy pigments go farther. T or F?

A

F

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10
Q

What is the equation for Rf?

A

distance moved by the pigment/ distance moved by the solvent front

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11
Q

List the pigment in order of increasing molecular weight?

A

Beta Carotene, Xanthophyll, Chlorophyll A, Chlorophyll B

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12
Q

What pigment will likely move the farthest?

A

Beta Carotene

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13
Q

What pigment has the greatest Rf value?

A

Beta Carotene

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14
Q

Small Rf indicated small molecular weight? T or F

A

F

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15
Q

What could prevent the movement on the solvent if you were to touch the chromatography paper?

A

lipids

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16
Q

What is DPIP

A

blue dye indicator

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17
Q

What is DPIP functioning as in this experiment?

A

electron acceptor

18
Q

What type of electron acceptor does DPIP mimic?

A

a final electron acceptor in the light dependent reaction

19
Q

What is the electron acceptor that DPIP replaces?

A

NADP+

20
Q

What color is DPIP in its oxidized form?

A

blue

21
Q

What color is DPIP in its reduced form?

A

colorless

22
Q

When loosing electrons a molecule is being (reduced/oxidized)?

A

oxidized

23
Q

When gaining electrons a molecule is being (reduced/oxidized)?

A

reduced

24
Q

When DPIP accepts electrons it is becoming (reduced/oxidized)?

A

reduced

25
Q

In what way does DPIP signify that photosynthesis is occurring?

A

Color change

26
Q

What is added to the spinach smoothie solution to keep it isotonic?

A

sucrose solution (0.5 M)

27
Q

What are the two versions of the spinach solution being observed?

A

boiled and unboiled

28
Q

What parts of the spinach are affected by boiling?

A

The proteins and the pigments in the thylakoid membrane

29
Q

What was the wavelength used in this experiment and what color does this signify?

A

635 nm (red)

30
Q

What was being “absorbed” in this experiment?

A

635 nm (red) light

31
Q

Why did absorbance decrease over time?

A

The electrons are being used for photosynthesis

32
Q

What color experienced transmittance the most through this experiment?

A

Blue

33
Q

What color did the solution turn as photosynthesis occurred (unboiled reaction)?

A

from blue to green

34
Q

What color did the solution turn for the boiled spinach?

A

from blue to blue (no color change)

35
Q

Why did the color of the unboiled solution fail to change?

A

wasn’t reduced (DPIP didn’t change)

36
Q

Was the rate of photosynthesis positive or negative for the unboiled spinach? Why?

A

Negative because electrons were used, therefore there were less and less available to reduce DPIP

37
Q

What was the expected rate of the boiled spinach?

A

zero

38
Q

What is the opposite of transmittance?

A

absorbance

39
Q

The light that makes it through the liquid chamber is (more/less intense)?

A

less intense

40
Q

What is a colorimeter measuring?

A

The amount of light transmitted through a sample

41
Q

The color of the solution is the color of the light that passes through. T or F?

A

T

42
Q

Would electrons be transferred to DPIP in the dark? Why?

A

No, light energy required to carry out light reactions and DPIP is the final electron acceptor in light reactions

Biology Laboratory (8 decks)

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