LAB

Question 1

Boiling flask

Answer 1

Florence flasks

Question 2

Conical flask

Answer 2

Erlenmeyer flasks

Question 3

Crucible is a small _____ cup made of a material that can withstand extreme temperatures.

Answer 3

clay

Question 4

A= a.b.c (Beer’s law)

Answer 4

A = absorbance
a = absorptivity
b = light path (in centimeters);
c = concentration of the absorbing compound (usually expressed in moles per liter).

Question 5

If you have higher number of wavelenght, you have ________ resolution.

Answer 5

smaller

Question 6

Spinal Cord (Medulla Spinalis)
Stain:
Shape:

Answer 6

Cresyl Violet
Pyramidal cell

Question 7

Sperm
Stain:
Shape:

Answer 7

Papanicolaou Stain
Tailed (Flagellar) Cell

Question 8

Ovarium
Stain:
Shape:

Answer 8

Hematoxylin-Eosin
Round cell

Question 9

Outer border of the organ is called cortex and inner part of this organ is called ________.

Answer 9

medulla

Question 10

Blood Smear
Stain:
Shape:

Answer 10

Giemsa
Round (Disk-Shaped)

Question 11

Lipid Droplets
Stain:

Answer 11

Osmium Tetroxide

Question 12

Lipids dissolve during routine of tissue preparation techniques so we use another technique called _______________.

Answer 12

osmium-impregnation

Question 13

Mitochondrion
Stain:

Answer 13

Iron-Hematoxylin

Question 14

Glycogen Granules
Stain:
Shape:

Answer 14

Best Carmine
Polygonal

Question 15

____ inhibits DNase activity by binding magnesium, so DNA can remain stable.

Answer 15

EDTA (Prevents blood coagulation as it binds Ca ++ ions)

Question 16

RNase inhibitors:

Answer 16

DEPC and RNaseZap

Question 17

pH is very important because ensuring that only RNA remains in upper phase occurs in _____ pH. In ____ pH, RNA and DNA remain in upper phase.

Answer 17

low, high