Lab 1&2 Spot test Flashcards
what are the steps to setting up a microscope
- clean lenses
- raise condenser
- move iris diaphragm left
- 4x lens in position
- light intensity 7
- power on
- change ocular lens distance
- mount
- look at lense and adjust with right eye, then left eye
- pencil test
what are the diameter sizes of each lens
4x = 4.40mm
10x = 1.75mm
40x = 0.44mm
how do we find the size on organism
diameter of field of view of objective / # of times it can fit across the field of view
what changes resolution
adjustment knob
what focuses light at level of specemen
condencer
2 prokaryotic organism groups are
Bacteria
Archaea
4 eukaryotic groups are
protists
fungi
plant
animal
what is the gelatenous outer covering of some bacterial cells
glycocalyx
what is the function of glycocalyx
Traps water and protects the bacterium from desiccation
what 4 structures should we look for to confirm its a living organism
cytoplasm
nucleus
plasma membrane
DNA
microscopy that focuses on thin planes and uses computer to make 3D images
SEM
ELectron microscopes have a higher ____ than light microscopes
resolving power
why do cells of onions lack green pigment
their plastid is a leucoplast which is colourless
what is stomata
pores
what is the purpose of stomata
gas exchange
