L8 Flashcards
what does hemizygous mean
eg. males only have one X chromosome
what is in situ hybridisation
involves the use of labelled DNA probes, which can be used to find the physical location of genes to specific chromosome regions
what has hampered the efforts of mapping the human genes
the inability to do test crosses, in addition each human family usually has a small number of progeny
what is somatic cell hybridisation
involves the fusion of different types of cells
what is a heterokaryon
combination of two nuclei
what does polyethylene glycol facilitate when mixed in a cell solution
the fusion their membranes
what does genetic mapping rely on
linkage and recombination
true or false, in situ hybridisation is a method for determining the chromosomal location of the gene through molecular analysis
true
what are types of DNA markers
minisatellites
microsatellites
SNPs (single nucleotide polymorphism
RFLPs (restriction fragment length polymorphism
what is a minisatellite
are markers based on the variation in the number of tandem repeats 15-100 bp long
what are microsatellites
are markers based on variation in the number of simpler sequences (2-6 bp), the most common is CA and its compliment GT
true or false, both mini and micro satellite loci have the same repeat unit but different number of repeat units
true
What are SNPs
Single nucleotide polymorphisms
- are positions in the genome where people differ in a single nucleotide base
What are RFLPs
restriction fragment length polymorphism, is a SNP that alters a restriction enzyme recognition site
what is a haplotype
a set of genetic determinants located on a single chromosome; particular set of alleles
