L6 Blood

Question 1

What is used to stain blood?

Answer 1

Wright stain

Question 2

Blood has cellular and extracellular elements. What are the cellular elements?

Answer 2

RBCs
WBCs
Platelets

Question 3

Blood has cellular and extracellular elements. What are the extra cellular elements?

Answer 3

Plasma (albumin + fibrinogen)

Question 4

What are the three layers blood separates into?

Answer 4

Plasma
Buffy coat
RBCs

Question 5

What are the contents of the buffy coat?

Answer 5

Leukocytes and platelets 1% of sample

Question 6

How many layers does blood separate into w/o anticoagulants?

Answer 6

2

Question 7

What layers does blood separate into w/o anticoagulants?

Answer 7

serum (w/ no fibrinogen) and blood clot

Question 8

What maintains the osmotic pressure inside the blood vessels?

Answer 8

plasma protein (albumin)

Question 9

T/F erythrocytes have nuclei

Answer 9

False

they are anucleate

Question 10

How long do erythrocytes stay in circulation?

Answer 10

120 days

Question 11

What condition causes excessive breakdown of RBCs leading to yellowing skin?

Answer 11

Jaundince

Question 12

What type of mutation leads to hereditary spherocytosis?

Answer 12

mutation of ankyrin proteins

Question 13

What type of mutation leads to hereditary elliptocytosis?

Answer 13

mutation of spectrin proteins

Question 14

What are the two major categories of leukocytes?

Answer 14

Granulocytes and Agranulocytes

Question 15

What are the three granulocytes?

Answer 15

neutrophils
basophils
eosinophils

Question 16

What are the two agranulocytes?

Answer 16

lymphocytes
monocytes

Question 17

Some cells can migrate out of the blood and into loose CT. Which cells become macrophages in tissue?

Answer 17

Monocytes

Question 18

Basophils in the blood are most related to what tissue cells?

Answer 18

Mast cells

Question 19

Which cells can recirculate from tissue to blood?

Answer 19

lymphocytes

Question 20

What cells are the most abundant leukocyte?

Answer 20

Neutrophils (PMNs)

Question 21

Primary, azurophilic granules are always…

Answer 21

lysosomes

Question 22

Tertiary granules or MMPs are only found in what cells?

Answer 22

Neutrophils

Question 23

What are the steps to neutrophil migration?

Answer 23

Rolling –> adhesion –> migration

Question 24

What cells have secondary granules that are histamine and vasoactive reagents?

Answer 24

basophils

Question 25

Basophils what are mostly characterized by?

Answer 25

bilobed S shape
Lots of granules stain dark
respond to allergic rxn

Question 26

What cells secondary granules contain Major basic protein and peroxidase?

Answer 26

eosinophils

Question 27

What white blood cells have granules that stain organge/pink/red?

Answer 27

eosinophils

Question 28

What is the fxn of eosinohphils?

Answer 28

response to parasitic infection

Question 29

When are eosinophils found in loose CT?

Answer 29

chronic inflammation
parasitic infection

Question 30

Which white blood cells are the largest?

Answer 30

monoytes

Question 31

What is the circulation time of monocytes?

Answer 31

1-3 days

Question 32

What white blood cells have a fxn of innate immune response?

Answer 32

monocytes

Question 33

What is the fxn of lymphocytes?

Answer 33

cell-mediated and humoral immune responses

Question 34

What is unique about lymphocytes nuclei?

Answer 34

it is large and stains dark

Question 35

What type of granules do platelets have?

Answer 35

fibrinogen
plasminogen
platelet-derived growth factor

Question 36

What cells are derived from megakaryocytes in the bone marrow?

Answer 36

platelets

Question 37

What are the steps of blood clot formation?

Answer 37

serotonin stops blood flow
ADP+Thromboxane form 1st plug
fibrinogen forms 2nd plug and flow retursn