L6 Flashcards
the process of destroying all forms of life, including spores and viruses
Sterilization
the process of destroying vegetative pathogens, but not necessarily spores or viruses
Disinfection
Physical methods
Heat, Filtration, Radiation
Moist heat
boiling, pasteurisation, autoclaving
Dry heat
hot air oven, incineration, flaming
Its disinfecting only, NOT sterilizing process
Boiling
Spores not killed by this method
Boiling
kills most of the pathogenic organisms, particularly viruses
and bacteria causing waterborne diseases
Boiling
Pasteurization Kills milk-borne vegetative pathogens such as:
Staphylococcus, Mycobacterium tuberculosis, Brucella, Salmonella,
Coxiella, Listeria
Are Spores killed in pasteurization
no
Slow pasteurization
heating at 63 to 65°C for 30 minutes
Rapid, high, or flash pasteurization
heating at 72°C for 15-20 seconds followed by rapid cooling
Sterilization under 15 pounds pressure and temperature of 121°C for 15 minutes
Autoclaving (Steam under pressure)
Most common method of sterilization for materials not damaged by heat or moisture
Autoclaving
Hot air oven
1700C for 30 minutes, 1600 C for 60 minutes, and 1500 C for 150 minutes