L3: Concentration Response Relationship Flashcards
• Draw typical “concentration vs response” and “log concentration vs response” curves with appropriately labelled axes • List some common responses to drugs and explain how they are measured • Distinguish between in vitro, in vivo and ex vivo measurements • Explain the term “EC50” and how it is measured • Distinguish between the terms “potency”, “potency ratio” and “relative potency” Explain the term “Therapeutic Index” and how it is measured
What does the concentration-response curve describe?
The relationship between drug concentration & response produced by the drug
What are the 3 main types of pharmalcological experiments?
In vivo
In vitro
Ex vivo
What is in vivo?
Inside of an living organism
What is in vitro?
Outside of an living organism
What is ex vivo?
Outside of an living organism, where cells, organs & tissues are removed, but then returned
How are drug effects studied in experiments in vitro?
Studied on a piece of tissue dissected from an animal & kept alive outside the body
How are drug effects studied in experiments in vivo?
Studied in the living animals e.g clinical trials
How are experiments in ex vivo ?
Animals are treated with the drug but then killed after a period of time
What concentration does most drugs act at?
1 x 10⁻⁶ to 1 x 10 ⁻¹²
What does a organ bath contain to keep tissue alive?
Physiological salt solution
What is the role of the force transducer?
Detect changes in tension by muscle
What is on the x and y axis of the concentration response curve?
X-axis: Representing the drug concentrations
Y-axis: Response (increase in force)
What does cumulative mean?
Increase in quantity by successive additions
Define potency
The concentration at which a drug is effective
How are doses expressed as?
Weight of drug per weight of animal
