L22 - Mol Biology and biotech

Question 1

GWAS (Genome wide association studies)

Answer 1

• Hypothesis-free way of identifying genome regions associated with a trait
• Linking phenotypic differences to genomic variations between populations

GWAS requires a large
panel with phenotypic
variation + high
throughput method to
phenotype

Question 2

Transcriptomics

Answer 2

Gene expression can be different:
•for different genes in the same family
• or the same gene in different
conditions/tissues

(Just because there is a gene, it doesn’t mean it is always expressed)

Question 3

Biotechnology in postharvest research

Answer 3

RNAi: Gene Silencing (Protection against viruses)
CRISPR: Gene Editing

Question 4

Agrobacterium - mediated transformation

Answer 4

Use of agrobacterium’s plasmid to transfer desired DNA fragment and make transgenic plants (See slide 23)

Question 5

Name of molecular biology sectors studying DNA, RNA, Proteins, Metabolites

Answer 5

DNA -> Genomics
RNA -> Transcriptomics
Proteins -> Proteomics
Metabolites -> Metabolomics

Answer 5

DNA -> Genomics
RNA -> Transcriptomics
Proteins -> Proteomics
Metabolites -> Metabolomics