L13 Euk Gene Expression Flashcards
1
Q
Level of regulation: transcription
A
cis and trans regulators, chromatic state will determine if/when genes are transcribed
2
Q
chromatin is found in which domain
A
Eukaryotes
3
Q
What are trans-acting factors?
A
They are regulatory proteins that
-have DNA binding domains, so can bind directly to DNA
-bind at transcription factor binding sites in cis-regulatory sequences
4
Q
What are cis-regulatory sequences?
A
DNA sequences where TFs bind, promoter, enhancer silencers
5
Q
TFs interest directly or indirectly with what?
A
RNA POL 2
6
Q
Mutate an enhancer, what happens to expression levels?
A
Reduced
7
Q
If you mutate a silencer, what happens to expression levels?
A
Increase expression levels
