L11 - HPV (3) Flashcards
Can HPV replicate in cells in suspension?
NO
It replicates as human skin cells differentiate
Can HPV vaccine be made from live virus?
NO - as cannot replicate in cells in suspension
What structural proteins does the HPV vaccine contain?
L1 (NOT L2)
What makes up the HPV vaccine?
Virus-like particles
Names of the 2 HPV vaccines?
Gardasil
Cervarix
Does the HPV capsid fit to normal capsid rules?
NO
No hexamers - BUT hexameric interactions with pentamers
Where is L2 located?
Holes inside the capsid
What cells is Cervarix made?
Insect cells
proprietary adjuvants
TLR4 ligands
What cells is Gardasil made in?
Yeast
Alum-based adjuvant
Why is Gardasil expensive?
VLPs have to be disassembled and reassembled to get rid of nucleic acid
Why only vaccinate girls against HPV?
Financial reasons
How is HPV detected and diagnosed?
Cytology - Pap smear
Serology
PCR primers designed in L1 ORF
In situ hybridisation
Immunocytochemistry
Assay - Digene - uses RNA probe cocktail detecting 13 HPVs
What is conisation?
excision of cone-shaped wedge from cervix including the transformation zone
What is the anti-viral agent used for HPV?
IMIQUIMOD
activates TLR7/8 mediated inflammatory response
Why is Imiquimod not the best treatment?
As soon as you remove the treatment it comes back
